Literature summary for 3.1.3.91 extracted from

Buschmann, J.; Moritz, B.; Jeske, M.; Lilie, H.; Schierhorn, A.; Wahle, E.
Identification of Drosophila and human 7-methyl GMP-specific nucleotidases (2013), J. Biol. Chem., 288, 2441-2451.

Search for more literature for 3.1.3.91

Activating Compound

Activating Compound	Comment	Organism	Structure
phosphate	stimulation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens
expression in S2 cell	Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	-	Drosophila melanogaster
KCl	-	Drosophila melanogaster
potassium acetate	-	Drosophila melanogaster
potassium phosphate	-	Drosophila melanogaster

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0078	-	N7-methyl-GMP	pH 7.5, 37°C	Homo sapiens
0.013	-	N7-methyl-GMP	pH 7.5, 25°C	Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	analytical ultracentrifugation	Homo sapiens
34400	-	34400, calculated	Homo sapiens
36000	-	PAGE	Drosophila melanogaster
36300	-	1 * 40000, SDS-PAGE, 1 * 36300, calculated	Drosophila melanogaster
40000	-	gel filtration	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	Q9W197	-	-
Homo sapiens	Q9H0P0	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Drosophila melanogaster	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme also efficiently dephosphorylates CMP, although with lower apparent affinity, Reaction of EC 3.1.3.5. UMP and the purine nucleotides are poor substrates	Drosophila melanogaster	?	-	?
additional information	enzyme also efficiently dephosphorylates CMP, Reaction of EC 3.1.3.5. UMP is a reasonable substrate, and the purine nucleotides are poor substrates	Homo sapiens	?	-	?
N7-methyl-GDP + H2O	-	Drosophila melanogaster	N7-methyl-guanosine + 2 phosphate	37fold less efficient than reaction with N7-methyl-GMP	?
N7-methyl-GDP + H2O	-	Homo sapiens	N7-methyl-guanosine + 2 phosphate	37fold less efficient than reaction with N7-methyl-GMP	?
N7-methyl-GMP + H2O	-	Drosophila melanogaster	N7-methyl-guanosine + phosphate	enzyme cleaves m7GMP to 7-methylguanosine and phosphate	?
N7-methyl-GMP + H2O	-	Homo sapiens	N7-methyl-guanosine + phosphate	enzyme cleaves m7GMP to 7-methylguanosine and phosphate	?

Subunits

Subunits	Comment	Organism
monomer	1 * 40000, SDS-PAGE, 1 * 36300, calculated	Drosophila melanogaster
monomer	34400, calculated	Homo sapiens

Synonyms

Synonyms	Comment	Organism
CG3362	-	Drosophila melanogaster
cytosolic 5'-nucleotidase 3	-	Homo sapiens
NT5C3A	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.24	-	N7-methyl-GMP	pH 7.5, 37°C	Homo sapiens
6.3	-	N7-methyl-GMP	pH 7.5, 25°C	Drosophila melanogaster

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Drosophila melanogaster

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.06	-	pH 7.5, 25°C	Drosophila melanogaster	potassium phosphate
0.52	-	pH 7.5, 25°C	Drosophila melanogaster	KCl
0.65	-	pH 7.5, 25°C	Drosophila melanogaster	potassium acetate
2	-	pH 7.5, 25°C	Drosophila melanogaster	AMP

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.3	-	N7-methyl-GMP	pH 7.5, 37°C	Homo sapiens
460	-	N7-methyl-GMP	pH 7.5, 25°C	Drosophila melanogaster

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Release 2023.1 (January 2023)