Literature summary for 3.1.3.9 extracted from

Jang, W.; Gomer, R.H.
A protein in crude cytosol regulates glucose-6-phosphatase activity in crude microsomes to regulate group size in Dictyostelium (2006), J. Biol. Chem., 281, 16377-16383.

Search for more literature for 3.1.3.9

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	in crude cytosols glucose-6-phosphatase activity has a positive correlation with counting factor accumulation. Molecules greater than 10 kDa from cells with low counting factor activity significantly increase activity of glucose-6-phosphatase in wild-type microsomes. In smlA- and wild-type cells, detergent treatment with Triton X-100 to allow direct access of D-glucose 6-phosphate to the catalytic subunit does not increase enzymatic activity. In low counting factor crude microsomes, detergent treatment increases glucose-6-phosphatase activity	Dictyostelium discoideum
additional information	secreted multipolypeptide complex called counting factor regulates one or more proteins greater than 10000 Da in crude cytosol that affect microsome-associated glucose-6-phosphatase activity	Dictyostelium discoideum

Application

Application	Comment	Organism
medicine	crucial enzyme involved in glucose homeostasis	Dictyostelium discoideum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	activity of glucose-6-phosphatase in crude microsomes from cells with high, normal, or low counting factor activity has a negative correlation with the amount of counting factor present in these cell lines. Molecules greater than 10 kDa from wild-type cells strongly repress activity of glucose-6-phosphatase in wild-type microsomes	Dictyostelium discoideum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	D-glucose 6-phosphate	cleared lysate of countin- cells with low activity of counting factor	Dictyostelium discoideum
0.1	-	D-glucose 6-phosphate	crude cytosol of cells with low counting factor	Dictyostelium discoideum
0.1	-	D-glucose 6-phosphate	crude cytosol of countin- cells with low activity of counting factor	Dictyostelium discoideum
0.1	-	D-glucose 6-phosphate	crude microsome of countin- cells with low activity of counting factor	Dictyostelium discoideum
0.18	-	D-glucose 6-phosphate	crude cytosol of wild-type	Dictyostelium discoideum
0.4	-	D-glucose 6-phosphate	crude cytosol of smlA- cells	Dictyostelium discoideum
0.4	-	D-glucose 6-phosphate	crude microsome of cells with disruption in smlA gene resulting in oversecretion of counting factor	Dictyostelium discoideum
0.65	-	D-glucose 6-phosphate	crude cytosol of wild-type cells	Dictyostelium discoideum
0.65	-	D-glucose 6-phosphate	crude microsome of wild-type	Dictyostelium discoideum
1.2	-	D-glucose 6-phosphate	crude cytosol of cells with disruption in smlA gene resulting in oversecretion of counting factor	Dictyostelium discoideum
2.1	-	D-glucose 6-phosphate	cleared lysate of wild-type	Dictyostelium discoideum
2.4	-	D-glucose 6-phosphate	ccleared lystae of cells with disruption in smlA gene resulting in oversecretion of counting factor	Dictyostelium discoideum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	activity of glucose-6-phosphatase in crude cytosol is positively correlated with the amount of secreted multipolypeptide complex called counting factor	Dictyostelium discoideum	5829	-
microsome	activity of glucose-6-phosphatase in crude microsome is negatively correlated with the amount of secreted multipolypeptide complex called counting factor	Dictyostelium discoideum	-	-

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	microsome	Dictyostelium discoideum	-
additional information	Ax4 cell, HDB7YA cell (smlA- cell), HDB2B/4 cell (cells with low counting factor)	Dictyostelium discoideum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate + H2O	-	Dictyostelium discoideum	D-glucose + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
glucose-6-phosphatase	-	Dictyostelium discoideum

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Release 2023.1 (January 2023)