BRENDA - Enzyme Database
show all sequences of 3.1.3.87

Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae

Myers, R.W.; Wray, J.W.; Fish, S.; Abeles, R.H.; J. Biol. Chem. 268, 24785-24791 (1993)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression in Klebsiella pneumoniae strain CG253 leading to 5-6fold increased activity compared to the parent strain
Klebsiella pneumoniae
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by phosphate
Klebsiella pneumoniae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Klebsiella pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25500
-
gel filtration
Klebsiella pneumoniae
28300
-
1 * 28300, SDS-PAGE
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
Klebsiella pneumoniae
-
1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
-
?
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
Klebsiella pneumoniae CG253
-
1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae CG253
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme by fractionation with help of protamine suzlfate, ammonium sulfate, and P-60, followed by anion exchange and hydrophobic interaction chromatography
Klebsiella pneumoniae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
overall pathway activity, purified enzyme, pH 7.5, 30°C
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
669181
Klebsiella pneumoniae
1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
-
-
?
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
669181
Klebsiella pneumoniae
1,2-dihydroxy-3-oxo-1-hexene + phosphate
product determination by NMR analysis. Conversion of 1,2-dihydroxy-3-keto-l-hexene to formate and 2-ketopentanoate occurs non-enzymatically
-
-
?
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
669181
Klebsiella pneumoniae CG253
1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
-
-
?
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
669181
Klebsiella pneumoniae CG253
1,2-dihydroxy-3-oxo-1-hexene + phosphate
product determination by NMR analysis. Conversion of 1,2-dihydroxy-3-keto-l-hexene to formate and 2-ketopentanoate occurs non-enzymatically
-
-
?
additional information
the phosphatase activity of the enzyme is highly specific, it shows no hydrolysis of phosphoenolpyruvate or 4-nitrophenyl phosphate
669181
Klebsiella pneumoniae
?
-
-
-
-
additional information
the phosphatase activity of the enzyme is highly specific, it shows no hydrolysis of phosphoenolpyruvate or 4-nitrophenyl phosphate
669181
Klebsiella pneumoniae CG253
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 28300, SDS-PAGE
Klebsiella pneumoniae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Klebsiella pneumoniae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Klebsiella pneumoniae strain CG253 leading to 5-6fold increased activity compared to the parent strain
Klebsiella pneumoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by phosphate
Klebsiella pneumoniae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Klebsiella pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25500
-
gel filtration
Klebsiella pneumoniae
28300
-
1 * 28300, SDS-PAGE
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
Klebsiella pneumoniae
-
1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
-
?
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
Klebsiella pneumoniae CG253
-
1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme by fractionation with help of protamine suzlfate, ammonium sulfate, and P-60, followed by anion exchange and hydrophobic interaction chromatography
Klebsiella pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
overall pathway activity, purified enzyme, pH 7.5, 30°C
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
669181
Klebsiella pneumoniae
1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
-
-
?
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
669181
Klebsiella pneumoniae
1,2-dihydroxy-3-oxo-1-hexene + phosphate
product determination by NMR analysis. Conversion of 1,2-dihydroxy-3-keto-l-hexene to formate and 2-ketopentanoate occurs non-enzymatically
-
-
?
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
669181
Klebsiella pneumoniae CG253
1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
-
-
?
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
669181
Klebsiella pneumoniae CG253
1,2-dihydroxy-3-oxo-1-hexene + phosphate
product determination by NMR analysis. Conversion of 1,2-dihydroxy-3-keto-l-hexene to formate and 2-ketopentanoate occurs non-enzymatically
-
-
?
additional information
the phosphatase activity of the enzyme is highly specific, it shows no hydrolysis of phosphoenolpyruvate or 4-nitrophenyl phosphate
669181
Klebsiella pneumoniae
?
-
-
-
-
additional information
the phosphatase activity of the enzyme is highly specific, it shows no hydrolysis of phosphoenolpyruvate or 4-nitrophenyl phosphate
669181
Klebsiella pneumoniae CG253
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 28300, SDS-PAGE
Klebsiella pneumoniae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Klebsiella pneumoniae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Klebsiella pneumoniae
General Information
General Information
Commentary
Organism
metabolism
Klebsiella pneumoniae converts 2,3-dioxo-5-methylthio-1-phosphopentane to formate, 2-oxo-4-methylthiobutyrate, and 3-methylthiopropionate. 2,3-Dioxo-1-phosphohexane, the desthio analogue of the naturally occurring diketone, can serve as alternate substrate in the methionine salvage pathway
Klebsiella pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
metabolism
Klebsiella pneumoniae converts 2,3-dioxo-5-methylthio-1-phosphopentane to formate, 2-oxo-4-methylthiobutyrate, and 3-methylthiopropionate. 2,3-Dioxo-1-phosphohexane, the desthio analogue of the naturally occurring diketone, can serve as alternate substrate in the methionine salvage pathway
Klebsiella pneumoniae
Other publictions for EC 3.1.3.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718388
Ashida
Was photosynthetic RuBisCO rec ...
Bacillus subtilis
Res. Microbiol.
156
611-618
2005
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-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706755
Ashida
A functional link between RuBi ...
Bacillus subtilis, Klebsiella pneumoniae
Science
302
286-290
2003
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1
-
-
-
-
-
-
1
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3
-
2
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-
-
-
-
-
-
-
5
-
3
1
-
-
-
1
-
-
-
-
-
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1
-
-
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-
-
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1
-
3
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
669181
Myers
Purification and characterizat ...
Klebsiella pneumoniae, Klebsiella pneumoniae CG253
J. Biol. Chem.
268
24785-24791
1993
-
-
1
-
-
-
1
-
-
1
2
2
-
3
-
-
1
-
-
-
1
-
6
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
2
2
-
-
-
1
-
-
1
-
6
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-