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Literature summary for 3.1.3.86 extracted from

  • Cestari, I.; McLeland-Wieser, H.; Stuart, K.
    Nuclear phosphatidylinositol 5-phosphatase is essential for allelic exclusion of variant surface glycoprotein genes in trypanosomes (2019), Mol. Cell. Biol., 39, e00395-18 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.86

Protein Variants

Protein Variants Comment Organism
D360A/N362A the mutations completely eliminate enzyme activity toward 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate and significantly decrease the dephosphorylation activity of the enzyme for 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0316
-
 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant enzyme D360A/N362A, at pH 7.5 and 25°C Trypanosoma brucei
0.0738
-
 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei
0.088
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Trypanosoma brucei 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O Trypanosoma brucei
-
 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Trypanosoma brucei
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protein A Mag Sepharose column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
 Trypanosoma brucei 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Trypanosoma brucei 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
phosphatidylinositol 5-phosphatase
-
 Trypanosoma brucei
PIP5Pase
-
 Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28.2
-
 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant enzyme D360A/N362A, at pH 7.5 and 25°C Trypanosoma brucei
57.5
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei
140.5
-
 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei

General Information

General Information Comment Organism
physiological function the enzyme's activity controls 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate binding by RAP1 and, thus, transcriptional silencing of variant surface glycoprotein genes Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
653
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei
892
-
 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant enzyme D360A/N362A, at pH 7.5 and 25°C Trypanosoma brucei
1900
-
 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei