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Literature summary for 3.1.3.86 extracted from

  • Le Coq, J.; Camacho-Artacho, M.; Velazquez, J.V.; Santiveri, C.M.; Gallego, L.H.; Campos-Olivas, R.; Doelker, N.; Lietha, D.
    Structural basis for interdomain communication in SHIP2 providing high phosphatase activity (2017), eLife, 6, e26640 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.86

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
using 100 mM Bis-Tris propane, pH 7.0, 0.2 M NaNO3, 20% (w/v) PEG3350, 0.025% (v/v) CH2Cl2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
F593D/L597D the mutations largely mimic the absence of the C2 domain Homo sapiens
R649A the mutation removes C2 domain effects on inositol 1,3,4,5-tetrakisphosphate but not 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate kinetics Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate wild type enzyme, at pH 7.0 and 23°C Homo sapiens
0.059
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate mutant enzyme R649A, at pH 7.0 and 23°C Homo sapiens
0.061
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate mutant enzyme F593D/L597D, at pH 7.0 and 23°C Homo sapiens
0.082
-
 Inositol 1,3,4,5-tetrakisphosphate mutant enzyme F593D/L597D, at pH 7.0 and 23°C Homo sapiens
0.098
-
 Inositol 1,3,4,5-tetrakisphosphate wild type enzyme, at pH 7.0 and 23°C Homo sapiens
0.126
-
 Inositol 1,3,4,5-tetrakisphosphate mutant enzyme R649A, at pH 7.0 and 23°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O15357
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
SH2-containing-inositol-5-phosphatase 2
-
 Homo sapiens
SHIP2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.69
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate wild type enzyme, at pH 7.0 and 23°C Homo sapiens
1.2
-
 Inositol 1,3,4,5-tetrakisphosphate mutant enzyme F593D/L597D, at pH 7.0 and 23°C Homo sapiens
1.32
-
 Inositol 1,3,4,5-tetrakisphosphate wild type enzyme, at pH 7.0 and 23°C Homo sapiens
1.35
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate mutant enzyme R649A, at pH 7.0 and 23°C Homo sapiens
1.64
-
 Inositol 1,3,4,5-tetrakisphosphate mutant enzyme R649A, at pH 7.0 and 23°C Homo sapiens
4.44
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate mutant enzyme F593D/L597D, at pH 7.0 and 23°C Homo sapiens