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Literature summary for 3.1.3.85 extracted from

  • Zheng, Q.; Jiang, D.; Zhang, W.; Zhang, Q.; Zhao, Q.; Jin, J.; Li, X.; Yang, H.; Bartlam, M.; Shaw, N.; Zhou, W.; Rao, Z.
    Mechanism of dephosphorylation of glucosyl-3-phosphoglycerate by a histidine phosphatase (2014), J. Biol. Chem., 289, 21242-21251.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of apo-, vanadate-bound, and phosphate-bound MtbGpgP are refined to 1.95 A, 2.3 A and 1.77 A, respectively. Enzyme consists of a single domain made up of a central beta-sheet flanked by alpha-helices on either side. The active site is located in a positively charged cleft situated above the central beta-sheet Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
E84Q mutant shows only 40% of catalytic activity compared to wild-type Mycobacterium tuberculosis
H159A mutation almost completely abolishes catalytic activity Mycobacterium tuberculosis
L209E mutation results in monomeric GpgP, rendering the enzyme incapable of dephosphorylation. Mutation almost completely abolishes catalytic activity Mycobacterium tuberculosis
N17A mutation almost completely abolishes catalytic activity Mycobacterium tuberculosis
Q23A mutant shows only 20% of catalytic activity compared to wild-type Mycobacterium tuberculosis
R10A mutation almost completely abolishes catalytic activity Mycobacterium tuberculosis
R60A mutation almost completely abolishes catalytic activity Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIC6
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Mycobacterium tuberculosis 4-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
MtbGpgP
-
 Mycobacterium tuberculosis
Rv2419c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Mycobacterium tuberculosis