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Literature summary for 3.1.3.85 extracted from

  • Costa, J.; Empadinhas, N.; Goncalves, L.; Lamosa, P.; Santos, H.; da Costa, M.S.
    Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii (2006), J. Bacteriol., 188, 1022-1030.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanococcoides burtonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 30°C Methanococcoides burtonii
0.1
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 30°C Thermus thermophilus
0.11
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 30°C Thermus thermophilus
0.27
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 50°C Methanococcoides burtonii
0.28
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 50°C Methanococcoides burtonii
0.36
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 50°C Thermus thermophilus
0.37
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 50°C Thermus thermophilus
0.79
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 70°C Thermus thermophilus
0.83
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 70°C Thermus thermophilus
0.9
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 90°C Thermus thermophilus
1.1
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 90°C Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. However, when 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate is the substrate, 3% activity is detected without cations, and the preferred metal ion with both substrates is Co2+ (10 mM). At 30°C, GpgP retains 37% of its enzyme activity towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate and 12% activity towards 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate in a reaction mixture without cations compared to the activity with 10 mM K+ and Co2+ Methanococcoides burtonii
K+ at 30°C, GpgP retains 37% of its enzyme activity towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate and 12% activity towards 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate in a reaction mixture without cations compared to the activity with 10 mM K+ and Co2+. Potassium chloride, in the concentration range of 10 to 100 mM, stimulates enzyme activity about 40% at 50°C, but only when 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate is the substrate. However, at 30°C, K+ has a stimulatory effect towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate, but not with 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate as the substrate Methanococcoides burtonii
Mg2+ at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. Mg2+ and, to a lesser extent, Ni2+ can replace Co2+ Methanococcoides burtonii
Mn2+ enzyme activity is not detected with Mn2+ when 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is used, while Mn2+ had a strong stimulatory effect (85% of the maximal activity with Co2+) with 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate Methanococcoides burtonii
additional information Ca2+, Sr2+, Ba2+, Cu2+, and Zn2+, have no effect on GpgP activity at any concentration Methanococcoides burtonii
Ni2+ at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. Mg2+ and, to a lesser extent, Ni2+ can replace Co2+ Methanococcoides burtonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O Methanococcoides burtonii synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O Thermus thermophilus synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanococcoides burtonii
-
-
-
Thermus thermophilus Q84B23
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Methanococcoides burtonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) Methanococcoides burtonii 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) Thermus thermophilus 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similar to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C Thermus thermophilus 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O the Vmax/Km-value for 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate Methanococcoides burtonii 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate + H2O the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similar to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C Thermus thermophilus 2-O-(alpha-D-mannopyranosyl)-D-glycerate + phosphate
-
 ?
2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate + H2O the Vmax/Km-value for 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii 2-O-(alpha-D-mannopyranosyl)-D-glycerate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GpgP
-
 Methanococcoides burtonii
GpgP
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii
60
-
 substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 55% of maximal activity, 70°C: about 50% of maximal activity, substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii
50 70 50°C: about 60% of maximal activity, 70°C: about 60% of maximal activity, substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 half-life: 198 h Methanococcoides burtonii
23
-
 half-life: 165 h Methanococcoides burtonii
50
-
 half-life: 12 min Methanococcoides burtonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii
6.5
-
 substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9 pH 4.5: about 40% of maximal activity, pH 9.0: about 45% of maximal activity, substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information the Vmax/Km-value for 2-O-(alpha-D-glucopyranisyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similat to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C Thermus thermophilus
additional information
-
 additional information the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii