Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-CMP | - |
Saccharomyces cerevisiae | |
3'-AMP | - |
Saccharomyces cerevisiae | |
ADP | - |
Saccharomyces cerevisiae | |
ADP-ribose | strong inhibition | Saccharomyces cerevisiae | |
AppA | - |
Saccharomyces cerevisiae | |
additional information | Poa1p is not inhibited by 5'3'-ADP , 5'-AMP, 2'-AMP, 2'-GMP, 3'-GMP, 3'-CMP, D-ribose-1-phosphate, D-ribose-5-phosphate, D-glucose-1-phosphate, D-fructose-6-phosphate, and glyceraldehyde-3-phosphate | Saccharomyces cerevisiae | |
NAD+ | - |
Saccharomyces cerevisiae | |
NADP+ | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
ADP-ribose 1''-phosphate | in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
estimated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-ribose 1''-phosphate + H2O | Saccharomyces cerevisiae | Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts | ADP-ribose + phosphate | - |
? | |
ADP-ribose 1''-phosphate + H2O | Saccharomyces cerevisiae NSY32 | Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts | ADP-ribose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38218 | - |
- |
Saccharomyces cerevisiae NSY32 | P38218 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon resin column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-ribose 1''-phosphate + H2O | Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts | Saccharomyces cerevisiae | ADP-ribose + phosphate | - |
? | |
ADP-ribose 1''-phosphate + H2O | Poa1p is highly specific for ADP-ribose 1''-phosphate | Saccharomyces cerevisiae | ADP-ribose + phosphate | - |
? | |
ADP-ribose 1''-phosphate + H2O | Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts | Saccharomyces cerevisiae NSY32 | ADP-ribose + phosphate | - |
? | |
ADP-ribose 1''-phosphate + H2O | Poa1p is highly specific for ADP-ribose 1''-phosphate | Saccharomyces cerevisiae NSY32 | ADP-ribose + phosphate | - |
? | |
additional information | Poa1p does not recognize pAp as substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Poa1p does not recognize pAp as substrate | Saccharomyces cerevisiae NSY32 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Appr1p phosphatase | - |
Saccharomyces cerevisiae |
ORF YBR022w | - |
Saccharomyces cerevisiae |
Poa1p | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
ADP-ribose 1''-phosphate | in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ADP-ribose | in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae | |
1 | - |
AppA | in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae | |
1.5 | - |
NAD+ | in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae | |
2 | - |
NADP+ | in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae | |
10 | - |
ADP | Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae | |
10 | - |
3'-AMP | Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae | |
10 | - |
2'-CMP | Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
ADP-ribose 1''-phosphate | in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C | Saccharomyces cerevisiae |