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Literature summary for 3.1.3.84 extracted from

  • Shull, N.P.; Spinelli, S.L.; Phizicky, E.M.
    A highly specific phosphatase that acts on ADP-ribose 1''-phosphate, a metabolite of tRNA splicing in Saccharomyces cerevisiae (2005), Nucleic Acids Res., 33, 650-660.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2'-CMP
-
Saccharomyces cerevisiae
3'-AMP
-
Saccharomyces cerevisiae
ADP
-
Saccharomyces cerevisiae
ADP-ribose strong inhibition Saccharomyces cerevisiae
AppA
-
Saccharomyces cerevisiae
additional information Poa1p is not inhibited by 5'3'-ADP , 5'-AMP, 2'-AMP, 2'-GMP, 3'-GMP, 3'-CMP, D-ribose-1-phosphate, D-ribose-5-phosphate, D-glucose-1-phosphate, D-fructose-6-phosphate, and glyceraldehyde-3-phosphate Saccharomyces cerevisiae
NAD+
-
Saccharomyces cerevisiae
NADP+
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
ADP-ribose 1''-phosphate in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
estimated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-ribose 1''-phosphate + H2O Saccharomyces cerevisiae Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts ADP-ribose + phosphate
-
?
ADP-ribose 1''-phosphate + H2O Saccharomyces cerevisiae NSY32 Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts ADP-ribose + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P38218
-
-
Saccharomyces cerevisiae NSY32 P38218
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon resin column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-ribose 1''-phosphate + H2O Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts Saccharomyces cerevisiae ADP-ribose + phosphate
-
?
ADP-ribose 1''-phosphate + H2O Poa1p is highly specific for ADP-ribose 1''-phosphate Saccharomyces cerevisiae ADP-ribose + phosphate
-
?
ADP-ribose 1''-phosphate + H2O Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts Saccharomyces cerevisiae NSY32 ADP-ribose + phosphate
-
?
ADP-ribose 1''-phosphate + H2O Poa1p is highly specific for ADP-ribose 1''-phosphate Saccharomyces cerevisiae NSY32 ADP-ribose + phosphate
-
?
additional information Poa1p does not recognize pAp as substrate Saccharomyces cerevisiae ?
-
?
additional information Poa1p does not recognize pAp as substrate Saccharomyces cerevisiae NSY32 ?
-
?

Synonyms

Synonyms Comment Organism
Appr1p phosphatase
-
Saccharomyces cerevisiae
ORF YBR022w
-
Saccharomyces cerevisiae
Poa1p
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
ADP-ribose 1''-phosphate in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 7
-
Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.03
-
ADP-ribose in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
1
-
AppA in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
1.5
-
NAD+ in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
2
-
NADP+ in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
10
-
ADP Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
10
-
3'-AMP Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
10
-
2'-CMP Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
ADP-ribose 1''-phosphate in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae