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Literature summary for 3.1.3.8 extracted from

  • Sandez Penidez, S.H.; Velasco Manini, M.A.; Gerez, C.L.; Rollan, G.C.
    Partial characterization and purification of phytase from Lactobacillus plantarum CRL1964 isolated from pseudocereals (2020), J. Basic Microbiol., 60, 787-798.
    View publication on PubMed
Search for more literature for 3.1.3.8

Activating Compound

Activating Compound Comment Organism Structure
acetate 10 mM, about 165% of initial activity Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 10 mM, about 60% of initial activity Lactiplantibacillus plantarum
Cu2+ 10 mM, about 80% of initial activity Lactiplantibacillus plantarum
DTT 10 mM, about 25% of initial activity Lactiplantibacillus plantarum
Fe2+ 10 mM, about 65% of initial activity Lactiplantibacillus plantarum
fluoride 10 mM, about 50% of initial activity Lactiplantibacillus plantarum
H2O2 10 mM, about 60% of initial activity Lactiplantibacillus plantarum
Ni2+ 10 mM, about 80% of initial activity Lactiplantibacillus plantarum
PMSF 10 mM, about 50% of initial activity Lactiplantibacillus plantarum
SDS 10 mM, about 60% of initial activity Lactiplantibacillus plantarum
sodium pyrophosphate 10 mM, about 20% of initial activity Lactiplantibacillus plantarum
Urea 10 mM, about 70% of initial activity Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum CRL1964
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified using ammonium sulfate precipitation Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Lactiplantibacillus plantarum 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Lactiplantibacillus plantarum CRL1964 4-nitrophenol + phosphate
-
 ?
adenosine 5'-diphosphate + H2O
-
 Lactiplantibacillus plantarum ? + phosphate
-
 ?
adenosine 5'-diphosphate + H2O
-
 Lactiplantibacillus plantarum CRL1964 ? + phosphate
-
 ?
D-glucose 1-phosphate + H2O
-
 Lactiplantibacillus plantarum glucose + phosphate
-
 ?
D-glucose 1-phosphate + H2O
-
 Lactiplantibacillus plantarum CRL1964 glucose + phosphate
-
 ?
phytate + H2O
-
 Lactiplantibacillus plantarum 1D-myo-inositol 1,2,3,4,5-pentakisphoshpate + phosphate
-
 ?
phytate + H2O
-
 Lactiplantibacillus plantarum CRL1964 1D-myo-inositol 1,2,3,4,5-pentakisphoshpate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 80
-
 Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Lactiplantibacillus plantarum

pH Range

pH Minimum pH Maximum Comment Organism
2 5.5
-
 Lactiplantibacillus plantarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6
-
 Lactiplantibacillus plantarum

Expression

Organism Comment Expression
Lactiplantibacillus plantarum presence of phytate induces phytase production, which is at its maximum at the end of the exponential phase up