Cloned (Comment) | Organism |
---|---|
gene phyC, DNA and amino acid determination and analysis, functional recombinant expression of thioredoxin-His-tagged enzyme from vector pET32a-PhyC and His-tagged enzyme from vector pET21a-PhyC in Escherichia coli cytoplasm. The concentration of the alkaline phytase expressed by pET32a is approximately 59% greater than that expressed by pET21, but its phytase activity is approximately 77% lower | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme contains a signal peptide | Bacillus subtilis | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Bacillus subtilis | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | Bacillus subtilis DR8886 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31097 | isolated from Dig Rostam hot mineral spring in Iran | - |
Bacillus subtilis DR8886 | O31097 | isolated from Dig Rostam hot mineral spring in Iran | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the mature peptide sequence lacked RHGXRXP, a conserved motif at the catalytic site of acidic phytases. The enzyme contains a signal peptide | Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
recombinant thioredoxin-His-tagged enzyme and His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme activity assay using color reagent containing 1.5% w/v ammonium molybdate, 5.5% v/v sulfuric acid solution, and 2.7% w/v ferrous sulfate | Bacillus subtilis | ? | - |
? | |
additional information | enzyme activity assay using color reagent containing 1.5% w/v ammonium molybdate, 5.5% v/v sulfuric acid solution, and 2.7% w/v ferrous sulfate | Bacillus subtilis DR8886 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | phytate | Bacillus subtilis | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis DR8886 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | phytate | Bacillus subtilis DR8886 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant thioredoxin-tagged enzyme, SDS-PAGE, x * 42000, recombinant nontagged enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
PhyC | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
additional information | the deduced amino acid sequence of phyC harbors a putative -35 and -10 sequences, a ribosomal binding site, and a transcription terminator | Bacillus subtilis |