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Literature summary for 3.1.3.8 extracted from

  • Higgins, N.F.; Crittenden, P.D.
    Phytase activity in lichens (2015), New Phytol., 208, 544-554 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
myo-inositol hexakisphosphate pH 2.5, 15°C Evernia prunastri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Evernia prunastri
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Lobaria pulmonaria
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Peltigera membranacea
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Ramalina fraxinea
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Bryoria fuscescens
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Pseudevernia furfuracea
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Ramalina calicaris
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Cladonia portentosa
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Hypogymnia physodes
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Parmelia saxatilis
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Platismatia glauca
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Ramalina farinacea
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Usnea subfloridana
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bryoria fuscescens
-
collected from The Halsary, Caithness, UK
-
Cladonia portentosa
-
collected from Migneit, Gwynedd, UK
-
Evernia prunastri
-
collected from the Derwent Valley, Derbyshire, UK, and from other sites subject to different rates of wet inorganic N (NO3- + NH4+) deposition, overview
-
Hypogymnia physodes
-
collected from Derwent Valley, Derbyshire, UK
-
Lobaria pulmonaria A0A2L2NKH0 Nostoc sp. is the Lobaria pulmonaria (5183) cyanobiont; collected from Lairg, Sutherland, UK
-
Parmelia saxatilis
-
collected from Derwent Valley, Derbyshire, UK
-
Peltigera membranacea A0A2L2ND21 Nostoc sp. is the Peltigera membranacea cyanobiont N6; collected from Beddgelert, Gwynedd, UK
-
Platismatia glauca
-
collected from Derwent Valley, Derbyshire, UK
-
Pseudevernia furfuracea
-
collected from The Halsary, Caithness, UK
-
Ramalina calicaris
-
collected from Lairg, Sutherland, UK
-
Ramalina farinacea
-
collected from Derwent Valley, Derbyshire, UK
-
Ramalina fraxinea
-
collected from Kildonan, Sutherland, UK
-
Usnea subfloridana
-
collected from Derwent Valley, Derbyshire, UK
-

Source Tissue

Source Tissue Comment Organism Textmining
thallus
-
Lobaria pulmonaria
-
thallus
-
Peltigera membranacea
-
thallus
-
Ramalina fraxinea
-
thallus
-
Bryoria fuscescens
-
thallus
-
Pseudevernia furfuracea
-
thallus
-
Ramalina calicaris
-
thallus
-
Cladonia portentosa
-
thallus
-
Hypogymnia physodes
-
thallus
-
Parmelia saxatilis
-
thallus
-
Platismatia glauca
-
thallus
-
Ramalina farinacea
-
thallus
-
Usnea subfloridana
-
thallus foliose thalli of fruticose lichens Evernia prunastri
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000017
-
about, crude enzyme, pH 2.5, 15°C Cladonia portentosa
0.0000083
-
about, crude enzyme, pH 3.2, 15°C Ramalina fraxinea
0.0000083
-
crude enzyme, pH 4.3, 15°C Peltigera membranacea
0.0000167
-
about, crude enzyme, pH 3.2, 15°C Hypogymnia physodes
0.0000167
-
about, crude enzyme, pH 3.4, 15°C Parmelia saxatilis
0.0000167
-
crude enzyme, pH 4.8, 15°C Lobaria pulmonaria
0.00002
-
about, crude enzyme, pH 4.8, 15°C Ramalina farinacea
0.000025
-
crude enzyme, pH 3.2, 15°C Ramalina calicaris
0.0000275
-
about, crude enzyme, pH 3.0, 15°C Platismatia glauca
0.000133
-
crude enzyme, pH 2.5, 15°C Pseudevernia furfuracea
0.000133
-
crude enzyme, pH 3.2, 15°C Usnea subfloridana
0.00015
-
crude enzyme, pH 2.5, 15°C Evernia prunastri
0.000183
-
crude enzyme, pH 2.5, 15°C Bryoria fuscescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also performs unspecific phosphomonoesterase (PME) activity with substrate 4-nitrophenyl phosphate. Phytase and PME activities in Evernia prunastri strongly co-vary among sites of sample collection with contrasting N deposition Evernia prunastri ?
-
?
myo-inositol hexakisphosphate + H2O
-
Evernia prunastri 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Lobaria pulmonaria 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Peltigera membranacea 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Ramalina fraxinea 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Bryoria fuscescens 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Pseudevernia furfuracea 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Ramalina calicaris 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Cladonia portentosa 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Hypogymnia physodes 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Parmelia saxatilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Platismatia glauca 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Ramalina farinacea 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Usnea subfloridana 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
NLP_0515
-
Lobaria pulmonaria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
highest activity at 30°C in a range of 5-30°C Evernia prunastri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 30 highest activity at 30°C in a range of 5-30°C, lowest at 5°C Evernia prunastri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
Evernia prunastri
2.5
-
-
Bryoria fuscescens
2.5
-
-
Pseudevernia furfuracea
2.5
-
-
Cladonia portentosa
3
-
-
Platismatia glauca
3.2
-
-
Ramalina fraxinea
3.2
-
-
Ramalina calicaris
3.2
-
-
Hypogymnia physodes
3.2
-
-
Usnea subfloridana
3.4
-
-
Parmelia saxatilis
4.3
-
-
Peltigera membranacea
4.8
-
-
Lobaria pulmonaria
4.8
-
-
Ramalina farinacea

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5 highest activity at pH 2.5, inactive at above pH 5.0 Evernia prunastri

General Information

General Information Comment Organism
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Evernia prunastri
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Lobaria pulmonaria
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Peltigera membranacea
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Ramalina fraxinea
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Bryoria fuscescens
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Pseudevernia furfuracea
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Ramalina calicaris
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Cladonia portentosa
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Hypogymnia physodes
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Parmelia saxatilis
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Platismatia glauca
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Ramalina farinacea
physiological function the phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface Usnea subfloridana