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Literature summary for 3.1.3.8 extracted from
- Recombinant HAP phytase of the thermophilic mold Sporotrichum thermophile expression of the codon-optimized phytase gene in Pichia pastoris and applications (2016), Mol. Biotechnol., 58, 137-147 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| butanol | slightly stimulates the phytase activity | Thermothelomyces heterothallicus |  |
| hexane | slightly stimulates the phytase activity | Thermothelomyces heterothallicus | |
| isoamyl alcohol | slightly stimulates the phytase activity | Thermothelomyces heterothallicus | |
| additional information | the chelating agent EDTA has no discernible effect on the activity of rSt-Phy | Thermothelomyces heterothallicus |
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | enzyme rSt-Phy is useful in the dephytinization of broiler feeds efficiently in simulated gut conditions of chick leading to the liberation of soluble inorganic phosphate with concomitant mitigation in anti-nutrient effects of phytates | Thermothelomyces heterothallicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene phyA, DNA and amino acid sequence determination and analysis, recombinant expression of the codon-optimized phytase gene in Pichia pastoris strain X-33, quantitative real-time PCR for gene copy number determination | Thermothelomyces heterothallicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Butanedione | - |
Thermothelomyces heterothallicus | |
| Al3+ | 23% inhibition at 5 mM | Thermothelomyces heterothallicus | |
| Co2+ | 10% inhibition at 5 mM | Thermothelomyces heterothallicus | |
| Cu2+ | 18% inhibition at 5 mM | Thermothelomyces heterothallicus | |
| Fe2+ | 12% inhibition at 5 mM | Thermothelomyces heterothallicus | |
| Fe3+ | 18% inhibition at 5 mM | Thermothelomyces heterothallicus | |
| guanidinium hydrochloride | - |
Thermothelomyces heterothallicus | |
| Mn2+ | 12% inhibition at 5 mM | Thermothelomyces heterothallicus | |
| additional information | the enzyme is resistant to both pepsin and trypsin. The chelating agent EDTA has no discernible effect on the activity of rSt-Phy | Thermothelomyces heterothallicus | |
| Na+ | 8% inhibition at 5 mM | Thermothelomyces heterothallicus | |
| potassium iodide | - |
Thermothelomyces heterothallicus | |
| Urea | - |
Thermothelomyces heterothallicus | |
| Woodward's reagent K | - |
Thermothelomyces heterothallicus | |
| Zn2+ | 61% inhibition at 5 mM | Thermothelomyces heterothallicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics and thermodynamics, overview | Thermothelomyces heterothallicus | |
| 0.147 | - |
myo-inositol hexakisphosphate | calcium phytate, pH 5.0, 60°C | Thermothelomyces heterothallicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Thermothelomyces heterothallicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activates | Thermothelomyces heterothallicus | |
| Mg2+ | activates | Thermothelomyces heterothallicus | |
| additional information | poor effect by Ca2+ at 1-5 mM | Thermothelomyces heterothallicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | Thermothelomyces heterothallicus | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces heterothallicus | V5M269 | i.e. Sporotrichum thermophile or Myceliophthora thermophila | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme is N-glycosylated, but also contains three potential O-glycosylation sites at positions 181, 185, and 268, it may be possible that the enzyme has one or more O-glycosylation. Deglycosylation using 10.0 U of endoglycosidase Hf (EndoHf) for 3 h at 37°C | Thermothelomyces heterothallicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris strain X-33 by ultrafiltration, anion exchange chromatography, dialysis, and gel filtration | Thermothelomyces heterothallicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the thermophilic mold Sporotrichum thermophile produces very low titers of phytase extracellularly in both solid state and submerged fermentations | Thermothelomyces heterothallicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0524 | - |
purified native enzyme, pH 5.0, 60°C | Thermothelomyces heterothallicus |
| 46 | - |
purified recombinant enzyme, pH 5.0, 60°C | Thermothelomyces heterothallicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme rSt-Phy also shows haloperoxidase activity with KBr, metavanadate, and H2O2, only histidine acid phosphatases with the active site sequence RHGXRXP can function as haloperoxidase, overview | Thermothelomyces heterothallicus | ? | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Thermothelomyces heterothallicus | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | substrate is calcium phytate | Thermothelomyces heterothallicus | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, recombinant enzyme, SDS-PAGE, x * 90000, native enzyme, SDS-PAGE | Thermothelomyces heterothallicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAP phytase | - |
Thermothelomyces heterothallicus |
| More | cf. EC 1.11.1.18 | Thermothelomyces heterothallicus |
| St-Phy | - |
Thermothelomyces heterothallicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
native and recombinant enzyme | Thermothelomyces heterothallicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 75 | activity range, profile overview | Thermothelomyces heterothallicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified enzyme, pH 5.0, half-life of recombinant enzyme is 6 h and of native enzyme 16 h | Thermothelomyces heterothallicus |
| 80 | - |
purified enzyme, pH 5.0, half-life of recombinant enzyme is 1 h and of native enzyme 1.5 h | Thermothelomyces heterothallicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1300 | - |
myo-inositol hexakisphosphate | calcium phytate, pH 5.0, 60°C | Thermothelomyces heterothallicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
native and recombinant enzyme | Thermothelomyces heterothallicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7.5 | activity range, profile overview | Thermothelomyces heterothallicus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8843.5 | - |
myo-inositol hexakisphosphate | calcium phytate, pH 5.0, 60°C | Thermothelomyces heterothallicus | |
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