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Literature summary for 3.1.3.8 extracted from

  • Tan, H.; Tang, J.; Li, X.; Liu, T.; Miao, R.; Huang, Z.; Wang, Y.; Gan, B.; Peng, W.
    Biochemical characterization of a psychrophilic phytase from an artificially cultivable morel Morchella importuna (2017), J. Microbiol. Biotechnol., 27, 2180-2189 .
    View publication on PubMed
Search for more literature for 3.1.3.8

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect by EDTA and EGTA at up to 20 mM Morchella importuna

Application

Application Comment Organism
agriculture since the enzyme degrades phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, it might be a promising candidate as a feed additive enzyme Morchella importuna

Cloned(Commentary)

Cloned (Comment) Organism
gene phyA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Pichia pastoris strain GS115 Morchella importuna

Inhibitors

Inhibitors Comment Organism Structure
Al3+ slight inhibition Morchella importuna
Ba2+
-
 Morchella importuna
Cu2+ slight inhibition Morchella importuna
Fe2+ slight inhibition Morchella importuna
Fe3+
-
 Morchella importuna
Hg2+
-
 Morchella importuna
Mn2+ slight inhibition Morchella importuna
Pb2+
-
 Morchella importuna
SDS 90% inhibition at 1%, complete inhibition at 10%, 10% inhibition at 0.1% Morchella importuna
Urea 40% inhibition at 100 mM, complete inhibition at 5 M Morchella importuna
Zn2+ slight inhibition Morchella importuna

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular no signal peptide is identified Morchella importuna
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61089
-
 mass spectrometry Morchella importuna

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Morchella importuna
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O Morchella importuna SCYDJ1A1
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Morchella importuna A0A2H4W727
-
-
Morchella importuna SCYDJ1A1 A0A2H4W727
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Morchella importuna
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2384.6
-
 purified enzyme, pH 6.5, 25°C Morchella importuna

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also shows moderate activity with 4-nitrophenyl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1,6-bisphosphate, and glucose 6-phosphate, and low activity with AMP, ADP, and ATP. The enzyme cleaves phytate in soybean meal, rapeseed meal, and corn meal Morchella importuna ?
-
 ?
additional information the enzyme also shows moderate activity with 4-nitrophenyl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1,6-bisphosphate, and glucose 6-phosphate, and low activity with AMP, ADP, and ATP. The enzyme cleaves phytate in soybean meal, rapeseed meal, and corn meal Morchella importuna SCYDJ1A1 ?
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Morchella importuna 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Morchella importuna SCYDJ1A1 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 61090, sequence calculation, 1 * 61089, mass spectrometry, 1 * 60000, SDS-PAGE Morchella importuna

Synonyms

Synonyms Comment Organism
MiPhyA
-
 Morchella importuna
PhyA
-
 Morchella importuna

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Morchella importuna

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 30 over 85% of maximal activity at 15-25°C, maximal activity at 25°C, sharp drop inactivity at above 30°C, over 50% of maximal activity at 0-5°C Morchella importuna

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 purified enzyme, pH 6.5, 180 min, 50% activity remaining Morchella importuna
45
-
 purified enzyme, pH 6.5, 50% activity remaining after 120 min, 20% after 180 min Morchella importuna
65
-
 purified enzyme, pH 6.5, 50% activity remaining after about 20 min, 10% after 60 min, inactivation after 120 min Morchella importuna

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Morchella importuna

pH Range

pH Minimum pH Maximum Comment Organism
2 7.5 activity range, profile overview. sharp drop in activity above pH 6.5 Morchella importuna

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 purified enzyme, 25°C, about 50% activity remaining after about 50 min, inactivation after 180 min Morchella importuna
6.5
-
 purified enzyme, 25°C, 240 min, 40% activity remaining Morchella importuna
11
-
 purified enzyme, 25°C, about 50% activity remaining after about 50 min, inactivation after 180 min Morchella importuna

General Information

General Information Comment Organism
evolution the enzyme belongs to the histidine acid phosphatase (HAP) family, it possesses typical conserved motifs of HAP phytases: RHGERFP in AA125-131 and HD in AA394-395 Morchella importuna