Cloned (Comment) | Organism |
---|---|
gene phy, DNA and amino acid sequence determination, analysis, and comparisons, cloning and expression in Escherichia coli strain DH5alpha | Bacillus amyloliquefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
LiCl | enzyme PHY US573 is extremely salt-tolerant preserving 80% and 95% of activity in the presence of 20 g/l of NaCl and LiCl, respectively | Bacillus amyloliquefaciens | |
additional information | the enzyme is resistant to bovine bile extract | Bacillus amyloliquefaciens | |
NaCl | enzyme PHY US573 is extremely salt-tolerant preserving 80% and 95% of activity in the presence of 20 g/l of NaCl and LiCl, respectively | Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.125 | - |
myo-inositol hexakisphosphate | pH 7.5, 70°C | Bacillus amyloliquefaciens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Bacillus amyloliquefaciens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, best at 1 mM | Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Bacillus amyloliquefaciens | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | Bacillus amyloliquefaciens US573 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | A0A0G4QKE4 | isolated from geother-mal soil located in Southern Tunisia | - |
Bacillus amyloliquefaciens US573 | A0A0G4QKE4 | isolated from geother-mal soil located in Southern Tunisia | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 6fold from cell culture medium of strain US573 by heat treatment and ammonium sulfate fractionation | Bacillus amyloliquefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
native extracellular enzyme, pH 7.5, 70°C | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is also able to dephosphorylate ATP and ADP with 95 and 29% of the activity compared to phytic acid, respectively | Bacillus amyloliquefaciens | ? | - |
? | |
additional information | the enzyme is also able to dephosphorylate ATP and ADP with 95 and 29% of the activity compared to phytic acid, respectively | Bacillus amyloliquefaciens US573 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus amyloliquefaciens | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | phytic acid | Bacillus amyloliquefaciens | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus amyloliquefaciens US573 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | phytic acid | Bacillus amyloliquefaciens US573 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PHY US573 | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus amyloliquefaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 85 | activity range, profile overview | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 100 | 50% and 62% activity remaining after 10 min at 100°C and 90°C, respectively | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | activity range, profile overview | Bacillus amyloliquefaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | purified enzyme, 37°C, shows good stability | Bacillus amyloliquefaciens |