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Literature summary for 3.1.3.8 extracted from
- Improved thermostability and enzyme activity of a recombinant phyA mutant phytase from Aspergillus niger N25 by directed evolution and site-directed mutagenesis (2018), Enzyme Microb. Technol., 108, 74-81 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F376Y | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
| K432R | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
| additional information | construction of three recombinant phyA mutant strains, i.e. PP-NPm-8, PP-NPep-6A and I44E/T252RPhyA, that show improved catalytic efficiency or thermostability compared to the wild-type strain. Variations of bond and electrostatic energy before and after substitution at Q172, T195, Q368, F376, and K432, half-life of thermal inactivation and kinetic parameters of PP-NPep-6A phytase and its mutants, predictions of residue interactions, overview | Aspergillus niger |
| Q172R | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
| Q172R/K432R | site-directed mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
| Q368E | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
| Q368E/K432R | site-directed mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
| T195L | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
| T195L/Q368E/F376Y | site-directed mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01073 | - |
myo-inositol hexakisphosphate | recombinant mutant Q368E/K432R, pH 5.5, 37°C | Aspergillus niger |  |
| 0.01547 | - |
myo-inositol hexakisphosphate | recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
| 0.01794 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R, pH 5.5, 37°C | Aspergillus niger | |
| 0.01927 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C | Aspergillus niger | |
| 0.02075 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
| 0.02189 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R, pH 5.5, 37°C | Aspergillus niger | |
| 0.02769 | - |
myo-inositol hexakisphosphate | recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C | Aspergillus niger | |
| 0.03073 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C | Aspergillus niger | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | Aspergillus niger | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | Aspergillus niger N25 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | P34754 | - |
- |
| Aspergillus niger N25 | P34754 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 286923 | - |
recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C | Aspergillus niger |
| 303715 | - |
recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C | Aspergillus niger |
| 438366 | - |
recombinant mutant Q172R/K432R, pH 5.5, 37°C | Aspergillus niger |
| 466688 | - |
recombinant mutant Q172R, pH 5.5, 37°C | Aspergillus niger |
| 467242 | - |
recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C | Aspergillus niger |
| 478761 | - |
recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger |
| 493308 | - |
recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger |
| 503217 | - |
recombinant mutant Q368E/K432R, pH 5.5, 37°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger N25 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-phytase B | - |
Aspergillus niger |
| Aspergillus niger N25 phytase | - |
Aspergillus niger |
| PhyB | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | activity range, profile overview | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.422 | - |
myo-inositol hexakisphosphate | recombinant mutant Q368E/K432R, pH 5.5, 37°C | Aspergillus niger | |
| 0.476 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C | Aspergillus niger | |
| 0.496 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C | Aspergillus niger | |
| 0.548 | - |
myo-inositol hexakisphosphate | recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
| 0.553 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R, pH 5.5, 37°C | Aspergillus niger | |
| 0.557 | - |
myo-inositol hexakisphosphate | recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C | Aspergillus niger | |
| 0.576 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R, pH 5.5, 37°C | Aspergillus niger | |
| 0.71 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7.5 | activity range, profile overview | Aspergillus niger |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.14 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C | Aspergillus niger | |
| 20.08 | - |
myo-inositol hexakisphosphate | recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C | Aspergillus niger | |
| 24.7 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C | Aspergillus niger | |
| 25.26 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R, pH 5.5, 37°C | Aspergillus niger | |
| 32.11 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R, pH 5.5, 37°C | Aspergillus niger | |
| 34.22 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
| 35.42 | - |
myo-inositol hexakisphosphate | recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
| 39.33 | - |
myo-inositol hexakisphosphate | recombinant mutant Q368E/K432R, pH 5.5, 37°C | Aspergillus niger | |
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