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Literature summary for 3.1.3.8 extracted from

  • Bandyopadhyay, D.; Das, K.; Sen, S.
    Purification of thermo and acid tolerant extracellular phytase from a new soil isolate of Amycolatopsis vancoresmycina S-12 (2017), Biocatal. Agricult. Biotechnol., 11, 302-306 .
No PubMed abstract available
Search for more literature for 3.1.3.8

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 40% inhibition at 10 mM Amycolatopsis vancoresmycina
Cu2+ 20% inhibition at 10 mM Amycolatopsis vancoresmycina
Fe2+ 30% inhibition at 10 mM Amycolatopsis vancoresmycina

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Amycolatopsis vancoresmycina
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates 17% at 10 mM Amycolatopsis vancoresmycina
additional information no or poor effect by Mn2+ and Zn2+ at 10 mM Amycolatopsis vancoresmycina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 native PAGE Amycolatopsis vancoresmycina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Amycolatopsis vancoresmycina
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O Amycolatopsis vancoresmycina S-12
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Amycolatopsis vancoresmycina
-
 isolated from soil, India
-
Amycolatopsis vancoresmycina S-12
-
 isolated from soil, India
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from culture medium 5.51fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography, and gel filtration, to near homogeneity Amycolatopsis vancoresmycina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Amycolatopsis vancoresmycina 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O phytate Amycolatopsis vancoresmycina 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Amycolatopsis vancoresmycina S-12 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O phytate Amycolatopsis vancoresmycina S-12 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE and native PAGE Amycolatopsis vancoresmycina
More peptide mass fingerprinting, MALDI-TOF mass spectrometry Amycolatopsis vancoresmycina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Amycolatopsis vancoresmycina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90 activity range, profile overview Amycolatopsis vancoresmycina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified native extracellular enzyme, completely stable up to Amycolatopsis vancoresmycina
70
-
 purified native extracellular enzyme, loss of 50% activity Amycolatopsis vancoresmycina
90
-
 purified native extracellular enzyme, loss of about 90% activity Amycolatopsis vancoresmycina
100
-
 purified native extracellular enzyme, inactivation Amycolatopsis vancoresmycina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Amycolatopsis vancoresmycina

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 purified native extracellular enzyme, most stable at Amycolatopsis vancoresmycina

pI Value

Organism Comment pI Value Maximum pI Value
Amycolatopsis vancoresmycina isoelectric focussing
-
 5.4

General Information

General Information Comment Organism
physiological function the enzyme promotes plant growth showing a significant effect, with an increase of 10% of root and 25% of shoot length, resulting in a 2.2fold increase in biomass Amycolatopsis vancoresmycina