Cloned (Comment) | Organism |
---|---|
- |
Hafnia alvei |
- |
Yersinia kristensenii |
Crystallization (Comment) | Organism |
---|---|
apo-enzyme, to 1.9 A resolution, and in complex with substrate analogue myo-inositiol hexakissulfate, to 1.59 A resolution. The substrate analogue is coordinated by residues Arg18, His19, Arg22, Thr25, Lys26, His128, Thr219, His306, Asp307 and a number of water molecules. The Nepsilon2 atom of the catalytic nucleophile, His19, lies 3.2 A from S6 of the analogue and is in-line with the S-O bond from the inositol ring. The OD1 atom of Asp307, the proton donor, forms an H-bond with the oxygen at position 6 of the inositol ring | Hafnia alvei |
in complex with phosphate, to 1.9 A resolution | Yersinia kristensenii |
Protein Variants | Comment | Organism |
---|---|---|
T308A | 5% of the specific activity of wild-type | Hafnia alvei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hafnia alvei | H9TUK5 | - |
- |
Yersinia kristensenii | H9TUK6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Hafnia alvei |
recombinant protein | Yersinia kristensenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate | Hafnia alvei | ? | - |
? | |
additional information | enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate | Yersinia kristensenii | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Hafnia alvei | DL-inositol 1,2,4,5,6-pentaphosphate + phosphate | 10-14% of product | ? | |
myo-inositol hexakisphosphate + H2O | - |
Yersinia kristensenii | DL-inositol 1,2,4,5,6-pentaphosphate + phosphate | 10-14% of product | ? |
Synonyms | Comment | Organism |
---|---|---|
PhyA | - |
Hafnia alvei |
PhyA | - |
Yersinia kristensenii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Yersinia kristensenii |
65 | - |
- |
Hafnia alvei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
melting temperature | Yersinia kristensenii |
70 | - |
melting temperature | Hafnia alvei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | - |
Hafnia alvei |
4 | 4.5 | - |
Yersinia kristensenii |