Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for activity. Activation of the enzyme involves a minimum of four Ca2+-binding sites and enzyme activation is mediated by cooperative interactions between Ca2+ and the enzyme or phytate. The apparent Km increases as the Ca2+ concentration is increased, and the apparent Vmax also increases | Pseudomonas sp. | |
Fe2+ | may partly substitute for Ca2+ in activation | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68852 | - |
x * 68852, calculated | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. BS10-3 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + 3 H2O | enzyme efficiently hydrolyzes Ca2+-phytate salts and yields myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products | Pseudomonas sp. | myo-inositol 2,4,6-trisphosphate + 3 phosphate | - |
? | |
myo-inositol hexakisphosphate + 3 H2O | enzyme efficiently hydrolyzes Ca2+-phytate salts and yields myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products | Pseudomonas sp. BS10-3 | myo-inositol 2,4,6-trisphosphate + 3 phosphate | - |
? | |
rice bran + H2O | natural phytate source | Pseudomonas sp. | ? + phosphate | - |
? | |
rice bran + H2O | natural phytate source | Pseudomonas sp. BS10-3 | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68852, calculated | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
beta-propeller phytase | - |
Pseudomonas sp. |
BPP | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
presence of 1 mM Ca2+, stable up to 55°C | Pseudomonas sp. |
60 | - |
rapid decrease in stability above | Pseudomonas sp. |
70 | - |
30 min, 20% residual activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas sp. |