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Literature summary for 3.1.3.8 extracted from

  • Nam, S.J.; Kim, Y.O.; Ko, T.K.; Kang, J.K.; Chun, K.H.; Auh, J.H.; Lee, C.S.; Lee, I.K.; Park, S.; Oh, B.C.
    Molecular and biochemical characteristics of beta-propeller phytase from marine Pseudomonas sp. BS10-3 and its potential application for animal feed additives (2014), J. Microbiol. Biotechnol., 24, 1413-1420.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity. Activation of the enzyme involves a minimum of four Ca2+-binding sites and enzyme activation is mediated by cooperative interactions between Ca2+ and the enzyme or phytate. The apparent Km increases as the Ca2+ concentration is increased, and the apparent Vmax also increases Pseudomonas sp.
Fe2+ may partly substitute for Ca2+ in activation Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68852
-
x * 68852, calculated Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. BS10-3
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + 3 H2O enzyme efficiently hydrolyzes Ca2+-phytate salts and yields myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products Pseudomonas sp. myo-inositol 2,4,6-trisphosphate + 3 phosphate
-
?
myo-inositol hexakisphosphate + 3 H2O enzyme efficiently hydrolyzes Ca2+-phytate salts and yields myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products Pseudomonas sp. BS10-3 myo-inositol 2,4,6-trisphosphate + 3 phosphate
-
?
rice bran + H2O natural phytate source Pseudomonas sp. ? + phosphate
-
?
rice bran + H2O natural phytate source Pseudomonas sp. BS10-3 ? + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 68852, calculated Pseudomonas sp.

Synonyms

Synonyms Comment Organism
beta-propeller phytase
-
Pseudomonas sp.
BPP
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
presence of 1 mM Ca2+, stable up to 55°C Pseudomonas sp.
60
-
rapid decrease in stability above Pseudomonas sp.
70
-
30 min, 20% residual activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pseudomonas sp.