Cloned (Comment) | Organism |
---|---|
expression of His-tagged phytase in Escherichia coli strain BL21(DE3) | Hahella chejuensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, best at 3 mM | Hahella chejuensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Hahella chejuensis | HcBPP preferentially recognizes its substrate and selectively hydrolyzes insoluble Ca2+-phytate salts at three phosphate group sites, yielding the final product, myo-inositol 2,4,6-trisphosphate, exhibiting also the 1- and 5-phytase activities. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+ | ? | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Hahella chejuensis | - |
? + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hahella chejuensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged phytase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Hahella chejuensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | HcBPP preferentially recognizes its substrate and selectively hydrolyzes insoluble Ca2+-phytate salts at three phosphate group sites, yielding the final product, myo-inositol 2,4,6-trisphosphate, exhibiting also the 1- and 5-phytase activities. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+ | Hahella chejuensis | ? | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Hahella chejuensis | ? + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-propeller phytase | - |
Hahella chejuensis |
BPP | - |
Hahella chejuensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
hydrolysis of Ca2+-phytate | Hahella chejuensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | the enzyme is stable at temperatures up to 55°C in the presence of 5 mM Ca2+, but the stability decreases dramatically above 65°C | Hahella chejuensis |
70 | - |
the enzyme retains more than 30% of its activity following incubation at 70°C for 30 min | Hahella chejuensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Hahella chejuensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | the pH profile of the enzyme contains peaks at pH 3.0 and pH 7.0, with the activity at pH 3.0 being approximately 70% of the peak activity at pH 7.0 | Hahella chejuensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | the enzyme retains more than 65% of the maximal activity in the pH range of pH 3.0-9.0. In addition, it is stable over the broad pH range of pH 3.0-10.0 | Hahella chejuensis |
General Information | Comment | Organism |
---|---|---|
physiological function | phytate is an antinutritional factor that influences the bioavailability of essential minerals by forming complexes with them and converting them into insoluble salts. beta-Propeller phytase hydrolyzes insoluble Ca2+-phytate salts and completely abrogates the ability of phytate to chelate metal ions, stoichiometry, affinity, and thermodynamics of these interactions by isothermal titration calorimetry, overview. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+ | Hahella chejuensis |