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Literature summary for 3.1.3.8 extracted from
- Transgenic microalgae expressing Escherichia coli AppA phytase as feed additive to reduce phytate excretion in the manure of young broiler chicks (2011), Appl. Microbiol. Biotechnol., 91, 553-563.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| engineering of Escherichia coli appA phytase gene into the chloroplast genome of the model microalga, Chlamydomonas reinhardtii strain CC-125, and isolation of homoplasmic plastid transformants. Catalytic activity of the recombinant AppA can be directly detected in the whole-cell lysate with 10 phytase units per gram dry matter at pH 4.5 and 37°C, method optimization, overview | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene APPA | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
10 phytase units per gram dry matter at pH 4.5 and 37°C for recombinant enzyme expressed in Chlamydomonas rheinhardtii | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AppA | - |
Escherichia coli |
| appA phytase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
recombinant enzyme | Escherichia coli |
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Release 2023.1 (January 2023)
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