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Literature summary for 3.1.3.8 extracted from

  • Shao, N.; Huang, H.; Meng, K.; Luo, H.; Wang, Y.; Yang, P.; Yao, B.
    Cloning, expression, and characterization of a new phytase from the phytopathogenic bacterium Pectobacterium wasabiae DSMZ 18074 (2008), J. Microbiol. Biotechnol., 18, 1221-1226.
    View publication on PubMed
Search for more literature for 3.1.3.8

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 113% of initial activity Pectobacterium wasabiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pectobacterium wasabiae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 25% of initial activity Pectobacterium wasabiae
Cr3+ 1 mM, 25% of initial activity Pectobacterium wasabiae
Cu2+ 1 mM, 3% of initial activity Pectobacterium wasabiae
Fe3+ 1 mM, 2.5% of initial activity Pectobacterium wasabiae
Hg2+ 1 mM, 0.6% of initial activity Pectobacterium wasabiae
SDS 1 mM, 0.4% of initial activity Pectobacterium wasabiae
Zn2+ 1 mM, 11% of initial activity Pectobacterium wasabiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 myo-inositol hexakisphosphate pH 5.0, 37°C Pectobacterium wasabiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, mature protein, calculated Pectobacterium wasabiae

Organism

Organism UniProt Comment Textmining
Pectobacterium wasabiae
-
 strain DSMZ 18074
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a putative signal sequence of 27 amino acids Pectobacterium wasabiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1072
-
 pH 5.0, 37°C Pectobacterium wasabiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Pectobacterium wasabiae 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, mature protein, calculated Pectobacterium wasabiae

Synonyms

Synonyms Comment Organism
AppA
-
 Pectobacterium wasabiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pectobacterium wasabiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, 70% residual activity Pectobacterium wasabiae
70
-
 2 min, loss of 90% of activity Pectobacterium wasabiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pectobacterium wasabiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 1 h, 37°C, more than 80% of initial activity retained Pectobacterium wasabiae

pI Value

Organism Comment pI Value Maximum pI Value
Pectobacterium wasabiae calculated
-
 5.5