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Literature summary for 3.1.3.8 extracted from
- Cloning and expression of Bacillus phytase gene (phy) in Escherichia coli and recovery of active enzyme from the inclusion bodies (2008), J. Appl. Microbiol., 105, 1128-1137.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Bacillus sp. (in: Bacteria) |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
myo-inositol hexakisphosphate | 37°C, pH 7.0 | Bacillus sp. (in: Bacteria) | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required both for refolding and activity | Bacillus sp. (in: Bacteria) | |
| additional information | enzyme refolded in presence of Cu2+, Fe2+, Mg2+, Co2+, Zn2+ or Mn2+, fails to show any enzyme activity even in the presence of Ca2+ in the reaction mix | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | A5A5J1 | - |
- |
| Bacillus sp. (in: Bacteria) DECSR1 | A5A5J1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, from inclusion bodies | Bacillus sp. (in: Bacteria) |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| Ca2+ is required for refolding. Enzyme refolded in the presence of one of the divalent cations, Cu2+, Fe2+, Mg2+, Co2+, Zn2+ or Mn2+, fails to show any enzyme activity even in the presence of Ca2+ in the reaction mix | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
37°C, pH 7.0 | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Bacillus sp. (in: Bacteria) | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Bacillus sp. (in: Bacteria) DECSR1 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | - |
Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Bacillus sp. (in: Bacteria) |
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