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Literature summary for 3.1.3.8 extracted from

  • Lei, X.G.; Kim, T.
    Expression of microbial phytases in yeast systems and characterization of the recombinant enzymes (2005), Methods Biotechnol., 17, 209-224.
No PubMed abstract available
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris (constitutive or inducible) and Saccharomyces cerevisiae (inducible) Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger P34752 3-phytase A precursor
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant enzyme expressed in yeast is highly glycosylated Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
partial Aspergillus niger

Synonyms

Synonyms Comment Organism
3-phytase A
-
 Aspergillus niger
PhyA phytase
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, stable, enzyme expressed in Saccharomyces cerevisiae or Pichia pastoris Aspergillus niger
65
-
 30 min, enzyme expressed in Saccharomyces cerevisiae loses about 30% of initial activity, enzyme expressed in Pichia pastoris loses about 45% of initial activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 enzyme expressed in Saccharomyces cerevisiae Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 pH 3.5: about 40% of maximal activity, pH 6.0: about 80% of maximal activity, pH 6.5: about 30% of maximal activity. enzyme expressed in Saccharomyces cerevisiae Aspergillus niger