Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.8 extracted from

  • Zinin, N.V.; Serkina, A.V.; Gelfand, M.S.; Shevelev, A.B.; Sineoky, S.P.
    Gene cloning, expression and characterization of novel phytase from Obesumbacterium proteus (2004), FEMS Microbiol. Lett., 236, 283-290.
    View publication on PubMed
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Obesumbacterium proteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 myo-inositol hexakisphosphate
-
 Obesumbacterium proteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Obesumbacterium proteus

Organism

Organism UniProt Comment Textmining
Obesumbacterium proteus Q6U677
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
310
-
-
 Obesumbacterium proteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1-phosphate + H2O poor substrate Obesumbacterium proteus D-fructose + phosphate
-
 ?
D-glucose 1-phosphate + H2O poor substrate Obesumbacterium proteus D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O poor substrate Obesumbacterium proteus D-glucose + phosphate
-
 ?
D-ribose 5-phosphate + H2O poor substrate Obesumbacterium proteus D-ribose + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Obesumbacterium proteus ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Obesumbacterium proteus

Synonyms

Synonyms Comment Organism
PhyA
-
 Obesumbacterium proteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50
-
 Obesumbacterium proteus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 30°C: about 55% of maximal activity, 65°C: about 40% of maximal activity, substrate: myo-inositol hexakisphosphate Obesumbacterium proteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, enzyme is highly resistant but loses its activity very rapidly under further increase of the temperature Obesumbacterium proteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 substrate: D-ribose 5-phosphate Obesumbacterium proteus
3.5
-
 substrate: D-glucose 1-phosphate, D-glucose 6-phosphate and D-fructose 1-phosphate Obesumbacterium proteus
4.9
-
 and a local pH-optimum at pH 3.4, substrate: myo-inositol hexakisphosphate Obesumbacterium proteus

pH Range

pH Minimum pH Maximum Comment Organism
2 6 pH 2.0: about 45% of maximal activiry, pH 6.0: about 50% of maximal activity, substrate: myo-inositol hexakisphosphate Obesumbacterium proteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.5 8 37°C, 0.2 M buffer solution, no significant change in activity after 24 h Obesumbacterium proteus