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Literature summary for 3.1.3.8 extracted from
- Gene cloning, expression and characterization of novel phytase from Obesumbacterium proteus (2004), FEMS Microbiol. Lett., 236, 283-290.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Obesumbacterium proteus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
myo-inositol hexakisphosphate | - |
Obesumbacterium proteus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Obesumbacterium proteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Obesumbacterium proteus | Q6U677 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 310 | - |
- |
Obesumbacterium proteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1-phosphate + H2O | poor substrate | Obesumbacterium proteus | D-fructose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | poor substrate | Obesumbacterium proteus | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | poor substrate | Obesumbacterium proteus | D-glucose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | poor substrate | Obesumbacterium proteus | D-ribose + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Obesumbacterium proteus | ? + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Obesumbacterium proteus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhyA | - |
Obesumbacterium proteus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | - |
Obesumbacterium proteus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | 30°C: about 55% of maximal activity, 65°C: about 40% of maximal activity, substrate: myo-inositol hexakisphosphate | Obesumbacterium proteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, enzyme is highly resistant but loses its activity very rapidly under further increase of the temperature | Obesumbacterium proteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
substrate: D-ribose 5-phosphate | Obesumbacterium proteus |
| 3.5 | - |
substrate: D-glucose 1-phosphate, D-glucose 6-phosphate and D-fructose 1-phosphate | Obesumbacterium proteus |
| 4.9 | - |
and a local pH-optimum at pH 3.4, substrate: myo-inositol hexakisphosphate | Obesumbacterium proteus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6 | pH 2.0: about 45% of maximal activiry, pH 6.0: about 50% of maximal activity, substrate: myo-inositol hexakisphosphate | Obesumbacterium proteus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | 8 | 37°C, 0.2 M buffer solution, no significant change in activity after 24 h | Obesumbacterium proteus |
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