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Literature summary for 3.1.3.8 extracted from

  • Hegeman, C.E.; Grabau, E.A.
    A novel phytase with sequence similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings (2001), Plant Physiol., 126, 1598-1608.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Glycine max

Inhibitors

Inhibitors Comment Organism Structure
ATP substrate inhibition above 5 mM Glycine max
additional information no substrate inhibition by 4-nitrophenyl phosphate up to 10 mM Glycine max
phytate substrate inhibition above 5 mM Glycine max
Polyphosphate substrate inhibition above 5 mM Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
myo-inositol hexakisphosphate
-
Glycine max
1.7
-
ATP
-
Glycine max
3.3
-
Polyphosphate
-
Glycine max
4.3
-
4-nitrophenyl phosphate
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
L. Merr.. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Glycine max

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon phytase synthesized early in germination persists for several days in the cotyledon Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
32000 OD355/mg Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Glycine max 4-nitrophenol + phosphate
-
?
ATP + H2O
-
Glycine max ADP + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Glycine max ? + phosphate
-
?
polyphosphate + H2O
-
Glycine max ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 70000-72000, SDS-PAGE Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
-
Glycine max

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
34 62 34°C: about 55% of maximal activity, 62°C: about 75% of maximal activity Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
10 min, stable up to Glycine max
70
-
10 min, 90% loss of activity Glycine max
80
-
10 min, complete loss of activity Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
3.8 5.8 pH 3.8: about 55% of maximal activity, pH 5.8: about 70% of maximal activity Glycine max