Cloned (Comment) | Organism |
---|---|
- |
Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | substrate inhibition above 5 mM | Glycine max | |
additional information | no substrate inhibition by 4-nitrophenyl phosphate up to 10 mM | Glycine max | |
phytate | substrate inhibition above 5 mM | Glycine max | |
Polyphosphate | substrate inhibition above 5 mM | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
myo-inositol hexakisphosphate | - |
Glycine max | |
1.7 | - |
ATP | - |
Glycine max | |
3.3 | - |
Polyphosphate | - |
Glycine max | |
4.3 | - |
4-nitrophenyl phosphate | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
L. Merr.. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Glycine max |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | phytase synthesized early in germination persists for several days in the cotyledon | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
32000 OD355/mg | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Glycine max | 4-nitrophenol + phosphate | - |
? | |
ATP + H2O | - |
Glycine max | ADP + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Glycine max | ? + phosphate | - |
? | |
polyphosphate + H2O | - |
Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000-72000, SDS-PAGE | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
- |
Glycine max |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | 62 | 34°C: about 55% of maximal activity, 62°C: about 75% of maximal activity | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, stable up to | Glycine max |
70 | - |
10 min, 90% loss of activity | Glycine max |
80 | - |
10 min, complete loss of activity | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | - |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.8 | 5.8 | pH 3.8: about 55% of maximal activity, pH 5.8: about 70% of maximal activity | Glycine max |