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Literature summary for 3.1.3.8 extracted from

  • Casey, A.; Walsh, G.
    Identification and characterization of a phytase of potential commercial interest (2004), J. Biotechnol., 110, 313-322.
    View publication on PubMed
Search for more literature for 3.1.3.8

Activating Compound

Activating Compound Comment Organism Structure
F- activates Rhizopus microsporus var. oligosporus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Rhizopus microsporus var. oligosporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0104
-
 myo-inositol hexakisphosphate pH 5.5 Rhizopus microsporus var. oligosporus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates Rhizopus microsporus var. oligosporus
Hg2+ activates Rhizopus microsporus var. oligosporus
Mg2+ activates Rhizopus microsporus var. oligosporus
Zn2+ activates Rhizopus microsporus var. oligosporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 1 * 120000, SDS-PAGE Rhizopus microsporus var. oligosporus
124000
-
 gel filtration Rhizopus microsporus var. oligosporus

Organism

Organism UniProt Comment Textmining
Rhizopus microsporus var. oligosporus
-
 ATCC 22959. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 30.5% total carbohydrate Rhizopus microsporus var. oligosporus

Purification (Commentary)

Purification (Comment) Organism
-
 Rhizopus microsporus var. oligosporus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhizopus microsporus var. oligosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O 89.6% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus 1-naphthol + phosphate
-
 ?
2-naphthyl phosphate + H2O 97.1% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus 2-naphthol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 59.6% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus 4-nitrophenol + phosphate
-
 ?
ADP + H2O 14.2% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus ?
-
 ?
AMP + H2O 25.0% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus adenosine + phosphate
-
 ?
ATP + H2O 22.3% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus ?
-
 ?
D-glucose 1-phosphate + H2O 92.0% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus D-glucose + phosphate
-
 ?
glycerol 2-phosphate + H2O 85.6% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus glycerol + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Rhizopus microsporus var. oligosporus ? + phosphate
-
 ?
phenyl phosphate + H2O 89.2% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus phenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 120000, SDS-PAGE Rhizopus microsporus var. oligosporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Rhizopus microsporus var. oligosporus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 75 37°C: about 65% of maximal activity, 75°C: about 45% of maximal activity Rhizopus microsporus var. oligosporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 about 25% loss of activity after 1 min, about 50% loss of activity after 2 min, about 65% loss of activity after 3 min Rhizopus microsporus var. oligosporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
 myo-inositol hexakisphosphate pH 5.5 Rhizopus microsporus var. oligosporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Rhizopus microsporus var. oligosporus

pH Range

pH Minimum pH Maximum Comment Organism
1.8 6 pH 1.8: about 45% of maximal activity, pH 6.0: about 45% of maximal activity Rhizopus microsporus var. oligosporus