Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mM-1 mM, slight inhibition | Lupinus albus | |
Cu2+ | 0.1 mM-1 mM, strong inhibition | Lupinus albus | |
F- | - |
Lupinus albus | |
Fe2+ | 0.1 mM-1 mM, strong inhibition | Lupinus albus | |
Fe3+ | 0.1 mM-1 mM, strong inhibition | Lupinus albus | |
Mn2+ | 0.1 mM-1 mM, slight inhibition | Lupinus albus | |
molybdate | - |
Lupinus albus | |
additional information | Mg2+ and Ca2+ show no effect at 0.1 mM-1 mM | Lupinus albus | |
phosphate | - |
Lupinus albus | |
vanadate | - |
Lupinus albus | |
wolframate | - |
Lupinus albus | |
Zn2+ | 0.1 mM-1 mM, strong inhibition | Lupinus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
myo-inositol hexakisphosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.123 | - |
4-nitrophenyl phosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.13 | - |
myo-inositol hexakisphosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.152 | - |
4-nitrophenyl phosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.176 | - |
4-nitrophenyl phosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.217 | - |
GTP | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.229 | - |
fructose 1,6-diphosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.3 | - |
myo-inositol hexakisphosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.302 | - |
D-glucose 6-phosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.315 | - |
AMP | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.319 | - |
AMP | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.359 | - |
D-fructose 1,6-diphosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.365 | - |
AMP | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.398 | - |
GTP | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.398 | - |
ADP | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.401 | - |
D-glucose 6-phosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.403 | - |
ADP | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.412 | - |
ATP | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.423 | - |
GTP | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.445 | - |
diphosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.451 | - |
ADP | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.478 | - |
D-fructose 6-phosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.49 | 2 | 1-naphthyl phosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.512 | - |
2-naphthyl phosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.516 | - |
1-naphthyl phosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.531 | - |
ATP | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.531 | - |
D-fructose 6-phosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.596 | - |
ATP | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.634 | - |
D-glucose 6-phosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.634 | - |
2-naphthyl phosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.653 | - |
2-naphthyl phosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.667 | - |
2-glycerophosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.676 | - |
D-fructose 6-phosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.691 | - |
diphosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.704 | - |
2-glycerophosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.718 | - |
1-naphthyl phosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.731 | - |
D-fructose 1,6-diphosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.74 | - |
O-phospho-L-serine | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.776 | - |
pyridoxal phosphate | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.8 | 11 | O-phospho-L-serine | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.812 | - |
diphosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus | |
0.876 | - |
pyridoxal phosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.881 | - |
2-glycerophosphate | pH 5.0, 35°C, phytase LP12 | Lupinus albus | |
0.891 | - |
O-phospho-L-serine | pH 5.0, 35°C, phytase LP11 | Lupinus albus | |
0.915 | - |
pyridoxal phosphate | pH 5.0, 35°C, phytase LP2 | Lupinus albus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | phytase LP11, LP12 and LP2 are not metalloenzymes | Lupinus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
phytase LP11 and LP12, gel filtration | Lupinus albus |
57000 | - |
1 * 57000, phytase LP11 and LP12, SDS-PAGE | Lupinus albus |
64000 | - |
1 * 64000, phytase LP2, SDS-PAGE | Lupinus albus |
65000 | - |
phytase LP2, gel filtration | Lupinus albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus albus | - |
var. amiga. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Purification (Comment) | Organism |
---|---|
three phytases LP11, LP12 and LP2 | Lupinus albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | 4-day-old | Lupinus albus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
498 | - |
phytase LP2 | Lupinus albus |
539 | - |
phytase LP11 | Lupinus albus |
607 | - |
phytase LP12 | Lupinus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Lupinus albus | 1-naphthol + phosphate | - |
? | |
2-glycerophosphate + H2O | - |
Lupinus albus | glycerate + phosphate | - |
? | |
2-naphthyl phosphate + H2O | - |
Lupinus albus | 2-naphthol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Lupinus albus | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | - |
Lupinus albus | ? | - |
? | |
AMP + H2O | - |
Lupinus albus | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Lupinus albus | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | - |
Lupinus albus | ? | - |
? | |
D-fructose 6-phosphate + H2O | - |
Lupinus albus | fructose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Lupinus albus | D-glucose + phosphate | - |
? | |
diphosphate + H2O | - |
Lupinus albus | 2 phosphate | - |
? | |
GTP + H2O | - |
Lupinus albus | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | hydrolyzes phytate in a stepwise manner | Lupinus albus | ? + phosphate | - |
? | |
O-phospho-L-serine + H2O | - |
Lupinus albus | L-serine + phosphate | - |
? | |
pyridoxal phosphate + H2O | - |
Lupinus albus | pyridoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 57000, phytase LP11 and LP12, SDS-PAGE | Lupinus albus |
monomer | 1 * 64000, phytase LP2, SDS-PAGE | Lupinus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
phytase LP11, LP12 and LP2 | Lupinus albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
90 min, no significant loss of activity at temperatures up ro, phytase LP11, LP12 and LP2 | Lupinus albus |
50 | - |
90 min, 15% loss of activity of phytase, phytase LP11, LP12 and LP2 | Lupinus albus |
60 | - |
90 min, 90 min, 79% loss of activity of phytase LP22, 92% loss of activity of phytase LP12 and 85% loss of activity of phytase LP2 | Lupinus albus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Lupinus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
phytase LP11, LP12 and LP2 | Lupinus albus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | enzyme is virtually inactive below pH 3.0 and above pH 8.0, phytase LP11, LP12 and LP2 | Lupinus albus |