Any feedback?
Literature summary for 3.1.3.8 extracted from
- Extracellular phytase (E.C. 3.1.3.8) from Aspergillus ficuum NRRL 3135: purification and characterization (1987), Prep. Biochem., 17, 63-91.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
myo-inositol hexakisphosphate | - |
Aspergillus ficuum |  |
| 0.265 | - |
4-nitrophenyl phosphate | - |
Aspergillus ficuum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus ficuum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
gel filtration | Aspergillus ficuum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus ficuum | - |
- |
- |
| Aspergillus ficuum NRRL 3135 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein | Aspergillus ficuum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus ficuum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus ficuum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 4.3, 5% v/v glycerol, 2 mM 2-mercaptoethanol, 1.7 mg/ml protein, stable for months | Aspergillus ficuum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Aspergillus ficuum | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Aspergillus ficuum NRRL 3135 | 4-nitrophenol + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum NRRL 3135 | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 85000-100000, SDS-PAGE | Aspergillus ficuum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
- |
Aspergillus ficuum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 61 | about 45% of maximal activity at 37°C and at 61°C | Aspergillus ficuum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 68 | - |
10 min, 60% loss of activity | Aspergillus ficuum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 104 | - |
4-nitrophenyl phosphate | - |
Aspergillus ficuum | |
| 220 | - |
myo-inositol hexakisphosphate | - |
Aspergillus ficuum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | - |
and a second optimum which is higher at pH 5.0-5.5 | Aspergillus ficuum |
| 5 | 5.5 | and a second optimum which is lower at pH 2.2 | Aspergillus ficuum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6 | pH 2.0: about 65% of maximal activity, pH 6.0: about 85% of maximal activity | Aspergillus ficuum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
