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Literature summary for 3.1.3.8 extracted from
- Purification and characterization of a phytase from Klebsiella terrigena (1997), Arch. Biochem. Biophys., 341, 201-206.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.1-1 mM, slight inhibition | Raoultella terrigena |  |
| Cu2+ | 0.1-1 mM, strong inhibition | Raoultella terrigena | |
| F- | - |
Raoultella terrigena | |
| Mn2+ | 0.1-1 mM, slight inhibition | Raoultella terrigena | |
| molybdate | - |
Raoultella terrigena | |
| phosphate | - |
Raoultella terrigena | |
| vanadate | - |
Raoultella terrigena | |
| Zn2+ | 0.1-1 mM, strong inhibition | Raoultella terrigena | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
myo-inositol hexakisphosphate | - |
Raoultella terrigena | |
| 6 | - |
NADP+ | - |
Raoultella terrigena | |
| 18 | - |
fructose 1,6-diphosphate | - |
Raoultella terrigena | |
| 20 | - |
glucose 6-phosphate | - |
Raoultella terrigena | |
| 55 | - |
ATP | - |
Raoultella terrigena | |
| 56 | - |
fructose 6-phosphate | - |
Raoultella terrigena | |
| 78 | - |
diphosphate | - |
Raoultella terrigena | |
| 90 | - |
ADP | - |
Raoultella terrigena | |
| 95 | - |
AMP | - |
Raoultella terrigena | |
| 110 | - |
Glycerol 2-phosphate | - |
Raoultella terrigena | |
| 120 | - |
4-nitrophenyl phosphate | - |
Raoultella terrigena | |
| 230 | - |
2-naphthyl phosphate | - |
Raoultella terrigena | |
| 250 | - |
1-naphthyl phosphate | - |
Raoultella terrigena | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Raoultella terrigena |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Raoultella terrigena | the enzyme is inducible under carbon limitation in the presence of myo-inositol hexakisphosphate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Raoultella terrigena | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Raoultella terrigena |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 205 | - |
- |
Raoultella terrigena |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl phosphate + H2O | - |
Raoultella terrigena | 1-naphthol + phosphate | - |
? | |
| 2-naphthyl phosphate + H2O | - |
Raoultella terrigena | 2-naphthol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Raoultella terrigena | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | - |
Raoultella terrigena | ? | - |
? | |
| AMP + H2O | - |
Raoultella terrigena | adenosine + phosphate | - |
? | |
| ATP + H2O | - |
Raoultella terrigena | ? | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Raoultella terrigena | D-fructose + phosphate | - |
? | |
| diphosphate + H2O | - |
Raoultella terrigena | 2 phosphate | - |
? | |
| fructose 1,6-diphosphate + H2O | - |
Raoultella terrigena | ? | - |
? | |
| Glucose 6-phosphate + H2O | - |
Raoultella terrigena | Glucose + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | - |
Raoultella terrigena | glycerol + phosphate | - |
? | |
| GTP + H2O | - |
Raoultella terrigena | ? | - |
? | |
| additional information | the enzyme is inducible under carbon limitation in the presence of myo-inositol hexakisphosphate | Raoultella terrigena | ? | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Raoultella terrigena | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| NADP+ + H2O | - |
Raoultella terrigena | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 40000, SDS-PAGE | Raoultella terrigena |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
- |
Raoultella terrigena |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
1 h, stable up to | Raoultella terrigena |
| 50 | - |
1 h, 20% loss of activity | Raoultella terrigena |
| 60 | - |
1 h, complete inactivation | Raoultella terrigena |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
glucose 6-phosphate | - |
Raoultella terrigena | |
| 2 | - |
1-naphthyl phosphate | - |
Raoultella terrigena | |
| 4 | - |
Glycerol 2-phosphate | - |
Raoultella terrigena | |
| 6 | - |
ATP | - |
Raoultella terrigena | |
| 6 | - |
fructose 6-phosphate | - |
Raoultella terrigena | |
| 6 | - |
2-naphthyl phosphate | - |
Raoultella terrigena | |
| 8 | - |
AMP | - |
Raoultella terrigena | |
| 9 | - |
ADP | - |
Raoultella terrigena | |
| 10 | - |
fructose 1,6-diphosphate | - |
Raoultella terrigena | |
| 11 | - |
diphosphate | - |
Raoultella terrigena | |
| 20 | - |
4-nitrophenyl phosphate | - |
Raoultella terrigena | |
| 46 | - |
NADP+ | - |
Raoultella terrigena | |
| 180 | - |
myo-inositol hexakisphosphate | - |
Raoultella terrigena | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Raoultella terrigena |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | - |
4°C, 24 h, 85% loss of activity | Raoultella terrigena |
| 2 | - |
4°C, 24 h, 45% loss of activity | Raoultella terrigena |
| 3 | 9 | 4°C, 10 days, no loss of activity | Raoultella terrigena |
| 5 | - |
30-50°C, no loss of activity | Raoultella terrigena |
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