Literature summary for 3.1.3.78 extracted from

Terebiznik, M.R.; Vieira, O.V.; Marcus, S.L.; Slade, A.; Yip, C.M.; Trimble, W.S.; Meyer, T.; Finlay, B.B.; Grinstein, S.
Elimination of host cell PtdIns(4,5)P(2) by bacterial SigD promotes membrane fission during invasion by Salmonella (2002), Nat. Cell Biol., 4, 766-773.

Cloned(Commentary)

Cloned (Comment)	Organism
Salmonella typhimurium SL1344 wild-type and deltaSigD mutant lacking SigD. Transfection of COS-7 and HeLa cells with SigD in mammalian expression vectors. Visualization of phosphatidylinositol 4,5-bisphosphate during Salmonella infection. Fusion protein of PH domain of phospholipase Cdelta and GFP (PLCdelta-PH-GFP) during invasion of HeLA cells by Salmonella (confocal fluorescence microscopy): phosphatidylinositol 4,5-bisphosphate is abundant near the tip but disappears from the basal regions. Role of SigD in phosphatidylinositol 4,5-bisphosphate elimination: focal disappearance of phosphatidylinositol 4,5-bisphosphate observed during invasion (wild-type) is diminished and delayed in the deltaSigD mutant (confocal fluorescence microscopy). SigD alters memebrane elasticity (atomic force microscopy, SigD transfected HeLa cells) and induces vacuole formation. SigD promotes membrane fission during invasion (SigD transfected HeLa cells)	Salmonella enterica subsp. enterica serovar Typhimurium

Cloned (Comment)

Organism

Salmonella typhimurium SL1344 wild-type and deltaSigD mutant lacking SigD. Transfection of COS-7 and HeLa cells with SigD in mammalian expression vectors. Visualization of phosphatidylinositol 4,5-bisphosphate during Salmonella infection. Fusion protein of PH domain of phospholipase Cdelta and GFP (PLCdelta-PH-GFP) during invasion of HeLA cells by Salmonella (confocal fluorescence microscopy): phosphatidylinositol 4,5-bisphosphate is abundant near the tip but disappears from the basal regions. Role of SigD in phosphatidylinositol 4,5-bisphosphate elimination: focal disappearance of phosphatidylinositol 4,5-bisphosphate observed during invasion (wild-type) is diminished and delayed in the deltaSigD mutant (confocal fluorescence microscopy). SigD alters memebrane elasticity (atomic force microscopy, SigD transfected HeLa cells) and induces vacuole formation. SigD promotes membrane fission during invasion (SigD transfected HeLa cells)

Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants	Comment	Organism
C462S	does not restore the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria	Salmonella enterica subsp. enterica serovar Typhimurium
K527A	restores the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria	Salmonella enterica subsp. enterica serovar Typhimurium
K530A	restores the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphatidylinositol 3,4,5-trisphosphate + H2O	Salmonella enterica subsp. enterica serovar Typhimurium	-	phosphatidylinositol 3,5-bisphosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	O30916	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphatidylinositol 3,4,5-trisphosphate + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium	phosphatidylinositol 3,5-bisphosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
SigD	-	Salmonella enterica subsp. enterica serovar Typhimurium