Literature summary for 3.1.3.78 extracted from

Ungewickell, A.; Hugge, C.; Kisseleva, M.; Chang, S.C.; Zou, J.; Feng, Y.; Galyov, E.E.; Wilson, M.; Majerus, P.W.
The identification and characterization of two phosphatidylinositol-4,5-bisphosphate 4-phosphatases (2005), Proc. Natl. Acad. Sci. USA, 102, 18854-18859.

Cloned(Commentary)

Cloned (Comment)	Organism
type I isozyme, DNA and amino acid sequence determination and analysis, transient transfection of functional enzyme to HeLa and COS-7 cells leading to increased EGFR degradation via increased phosphatidylinositol-5-phosphate content, functional overexpression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus infection system, stable inducible expression of labeled isozyme I in HEK293 cells	Homo sapiens
type II isozyme, DNA and amino acid sequence determination and analysis, transient transfection of functional enzyme to HeLa and COS-7 cells leading to increased EGFR degradation via increased phosphatidylinositol-5-phosphate content, functional overexpression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus infection system	Homo sapiens

Cloned (Comment)

Organism

type I isozyme, DNA and amino acid sequence determination and analysis, transient transfection of functional enzyme to HeLa and COS-7 cells leading to increased EGFR degradation via increased phosphatidylinositol-5-phosphate content, functional overexpression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus infection system, stable inducible expression of labeled isozyme I in HEK293 cells

Homo sapiens

type II isozyme, DNA and amino acid sequence determination and analysis, transient transfection of functional enzyme to HeLa and COS-7 cells leading to increased EGFR degradation via increased phosphatidylinositol-5-phosphate content, functional overexpression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus infection system

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	expression of isozyme I siRNA in HeLa cells does not affect phosphatidylinositol-4,5-bisphosphate levels or EGFR degradation	Homo sapiens
additional information	expression of isozyme II siRNA in HeLa cells does not affect phosphatidylinositol-4,5-bisphosphate levels or EGFR degradation	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endosome membrane	late	Homo sapiens	10008	-
lysosomal membrane	late	Homo sapiens	5765	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the enzyme affects epithelial growth factor receptor EGFR degradation upon epithelial growth factor EGF stimulation via phosphatidylinositol-5-phosphate, overview	?	-	?
phosphatidylinositol-4,5-bisphosphate + H2O	Homo sapiens	-	phosphatidylinositol-5-phosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q86T03	two isozyme I	-
Homo sapiens	Q8N4L2	isozyme II	-

Purification (Commentary)

Purification (Comment)	Organism
FLAG-tagged isozyme I from Sf9 insect cells by anti-FLAG immunoaffinity chromatography	Homo sapiens
FLAG-tagged isozyme II from Sf9 insect cells by anti-FLAG immunoaffinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal gland	-	Homo sapiens	-
brain	-	Homo sapiens	-
liver	-	Homo sapiens	-
additional information	isozyme II is ubiquitously expressed, comparison of expression patterns of isozymes I and II, overview	Homo sapiens	-
salivary gland	-	Homo sapiens	-
spleen	-	Homo sapiens	-
thymus	-	Homo sapiens	-
thyroid gland	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme affects epithelial growth factor receptor EGFR degradation upon epithelial growth factor EGF stimulation via phosphatidylinositol-5-phosphate, overview	Homo sapiens	?	-	?
phosphatidylinositol-4,5-bisphosphate + H2O	-	Homo sapiens	phosphatidylinositol-5-phosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
phosphatidylinositol-4,5-bisphosphate 4-phosphatase I	-	Homo sapiens
phosphatidylinositol-4,5-bisphosphate 4-phosphatase II	-	Homo sapiens
type I PtdIns-4,5-P2 4-Ptase	-	Homo sapiens
type II PtdIns-4,5-P2 4-Ptase	-	Homo sapiens