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Literature summary for 3.1.3.77 extracted from
- Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae (1993), J. Biol. Chem., 268, 24785-24791.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0071 | - |
2-hydroxy-3-keto-1-phospho-1-hexene | - |
Klebsiella pneumoniae |  |
| 0.077 | - |
2,3-Diketo-1-phosphohexane | - |
Klebsiella pneumoniae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25500 | - |
gel filtration | Klebsiella pneumoniae |
| 27000 | - |
calculated from sequence | Klebsiella pneumoniae |
| 28300 | - |
SDS-PAGE | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
CG253 | - |
| Klebsiella pneumoniae CG253 | - |
CG253 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 100fold purified to homogeneity in 10% yield, SDS-PAGE, amino acid sequence analysis | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21 | - |
purified enzyme by phenyl-Superose column | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-diketo-1-phosphohexane + H2O | including two intermediates, 2-hydroxy-3-keto-1-phospho-1-hexene and 1,2-dihydroxy-3-keto-1-hexene | Klebsiella pneumoniae | 2-ketopentanoate + formate + glyoxylate + butyrate | - |
? | |
| 2,3-diketo-1-phosphohexane + H2O | including two intermediates, 2-hydroxy-3-keto-1-phospho-1-hexene and 1,2-dihydroxy-3-keto-1-hexene | Klebsiella pneumoniae CG253 | 2-ketopentanoate + formate + glyoxylate + butyrate | - |
? | |
| 2-hydroxy-3-keto-1-phospho-1-hexene + H2O | - |
Klebsiella pneumoniae | 2-ketopentanoate + formate + glyoxylate + butyrate | - |
? | |
| 2-hydroxy-3-keto-1-phospho-1-hexene + H2O | - |
Klebsiella pneumoniae CG253 | 2-ketopentanoate + formate + glyoxylate + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Klebsiella pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| E-1 | - |
Klebsiella pneumoniae |
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