Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.77 extracted from

  • Wang, H.; Pang, H.; Bartlam, M.; Rao, Z.
    Crystal Structure of Human E1 Enzyme and its Complex with a Substrate Analog Reveals the Mechanism of its Phosphatase/Enolase Activity (2005), J. Mol. Biol., 348, 917-926.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the bacterial expression vector pET28a with a His tag on the C-terminus. Recombinant plasmid overexpressed in Escherichia coli strain BL21 (DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure to 1.7 A resolution of MASA and its complex with a substrate analog, by hanging-drop vapor-diffusion technique Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ three magnesium ions, one is buried in the active-site pocket in the interface between the two domains, where it serves as a cofactor for the enzyme activity. The other two are located on the surface of the molecule and are supposedly helpful for packing of crystals. Magnesium ions originate from the reservoir solution during crystallization and are absolutely required for the enzyme stability and activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UHY7
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni2+-chelating affinity column, gel filtration and anion-exchange chromatography, more than 90% pure Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 20 mM HEPES buffer, pH 7.5 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1-oxo-heptyl)-phosphonic acid + H2O substrate analog, in complex with MASA it sits in the cleft formed by the conserved residues in the active site Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
enolase-phosphatase
-
Homo sapiens
MASA
-
Homo sapiens