Cloned (Comment) | Organism |
---|---|
into the bacterial expression vector pET28a with a His tag on the C-terminus. Recombinant plasmid overexpressed in Escherichia coli strain BL21 (DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structure to 1.7 A resolution of MASA and its complex with a substrate analog, by hanging-drop vapor-diffusion technique | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | three magnesium ions, one is buried in the active-site pocket in the interface between the two domains, where it serves as a cofactor for the enzyme activity. The other two are located on the surface of the molecule and are supposedly helpful for packing of crystals. Magnesium ions originate from the reservoir solution during crystallization and are absolutely required for the enzyme stability and activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UHY7 | - |
- |
Purification (Comment) | Organism |
---|---|
by Ni2+-chelating affinity column, gel filtration and anion-exchange chromatography, more than 90% pure | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, 20 mM HEPES buffer, pH 7.5 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1-oxo-heptyl)-phosphonic acid + H2O | substrate analog, in complex with MASA it sits in the cleft formed by the conserved residues in the active site | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
enolase-phosphatase | - |
Homo sapiens |
MASA | - |
Homo sapiens |