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Literature summary for 3.1.3.76 extracted from

  • Tran, K.L.; Aronov, P.A.; Tanaka, H.; Newman, J.W.; Hammock, B.D.; Morisseau, C.
    Lipid sulfates and sulfonates are allosteric competitive inhibitors of the N-terminal phosphatase activity of the mammalian soluble poxide hydrolase (2005), Biochemistry, 44, 12179-12187.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.76

Inhibitors

Inhibitors Comment Organism Structure
(9E)-12-sulfonooxy-9-octadecenoic acid IC50 is 0.0097 mM Homo sapiens
(9Z)-12-sulfonooxy-9-octadecenoic acid IC50 is above 0.1 mM Homo sapiens
10-(sulfooxy)octadecanoic acid IC50 is 0.0059 mM Homo sapiens
4-nitrophenyl sulfate IC50 is above 0.1 mM Homo sapiens
9-hydroxy-10-(sulfooxy)octadecanoic acid IC50 is 0.0175 mM Homo sapiens
alpha-hydroxyfarnesyl phosphonic acid IC50 is 0.073 mM Homo sapiens
alpha-sulfostearic acid IC50 is 0.0096 mM Homo sapiens
D-galactose 6-sulfate IC50 is above 0.1 mM Homo sapiens
dibenzyl phosphonate IC50 is above 0.1 mM Homo sapiens
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate IC50 is above 0.1 mM Homo sapiens
diethyl 4-methylbenzyl phosphonate IC50 is above 0.1 mM Homo sapiens
diethyl allyl phosphonate IC50 is above 0.1 mM Homo sapiens
diethyl benzoylphosphonate IC50 is above 0.1 mM Homo sapiens
diethyl cyclopropyl methylphosphonate IC50 is above 0.1 mM Homo sapiens
diethyl ethylthiomethyl phosphonate IC50 is above 0.1 mM Homo sapiens
diethyl trans-cinnamyl phosphonate IC50 is above 0.1 mM Homo sapiens
diethyl vinylphosphonate IC50 is above 0.1 mM Homo sapiens
dimethyl 2-oxoheptyl phosphonate IC50 is above 0.1 mM Homo sapiens
dioctyl phenyl phosphonate IC50 is 0.013 mM Homo sapiens
dodecyl phosphonic acid IC50 is 0.040 mM Homo sapiens
Estrone 3-sulfate IC50 is above 0.1 mM Homo sapiens
L-ascorbic acid 2-sulfate IC50 is above 0.1 mM Homo sapiens
additional information inhibition mechanism of sulfates, sulfonates, and phosphonates Homo sapiens
N-acetyl-D-galactosamine 4-sulfate IC50 is above 0.1 mM Homo sapiens
octadecan-9-yl sulfate IC50 is 0.0047 mM Homo sapiens
Sodium dodecyl sulfate IC50 is 0.0052 mM Homo sapiens
sodium dodecyl sulfonate IC50 is 0.0037 mM Homo sapiens
taurocholic acid IC50 is above 0.1 mM Homo sapiens
taurolithocholic acid 3-sulfate IC50 is 0.005 mM Homo sapiens
tetraisopropyl methylenediphosphonate IC50 is above 0.1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens
0.0036
-
 farnesyl diphosphate pH 7.0, 30°C, recombinant enzyme Homo sapiens
0.0036
-
 geranylgeranyl diphosphate pH 7.0, 30°C, recombinant enzyme Homo sapiens
0.0036
-
 farnesyl monophosphate pH 7.0, 30°C, recombinant enzyme Homo sapiens
0.0036
-
 2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole phosphate pH 7.0, 30°C, recombinant enzyme Homo sapiens
0.021
-
 threo-9,10-phosphonooxyoctadecanoic acid pH 7.0, 30°C, recombinant enzyme Homo sapiens
0.21
-
 4-methylumbelliferyl phosphate pH 7.0, 30°C, recombinant enzyme Homo sapiens
1.6
-
 4-nitrophenyl phosphate pH 7.0, 30°C, recombinant enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently, reaction mechanism Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9E)-12-sulfonooxyoctadec-9-enoic acid + H2O
-
 Homo sapiens (9E)-12-hydroxyoctadec-9-enoic acid + sulfate
-
 ?
(9Z)-12-sulfonooxyoctadec-9-enoic acid + H2O
-
 Homo sapiens (9Z)-12-hydroxyoctadec-9-enoic acid + sulfate
-
 ?
10-sulfonooxy-octadecanoic acid + H2O
-
 Homo sapiens 10-hydroxyoctadecanoic acid + phosphate
-
 ?
2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole phosphate + H2O i.e. attophos Homo sapiens 2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole + phosphate
-
 ?
4-methylumbelliferyl phosphate + H2O
-
 Homo sapiens 4-methylumbelliferone + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Homo sapiens 4-nitrophenol + phosphate
-
 ?
farnesyl diphosphate + H2O
-
 Homo sapiens farnesol + phosphate
-
 ?
farnesyl phosphate + H2O
-
 Homo sapiens farnesol + phosphate
-
 ?
geranyl diphosphate + H2O
-
 Homo sapiens geraniol + phosphate
-
 ?
geranylgeranyl diphosphate + H2O
-
 Homo sapiens geranylgeraniol + phosphate
-
 ?
threo-9,10-phosphonooxyoctadecanoic acid + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10 Homo sapiens

Synonyms

Synonyms Comment Organism
EPXH2
-
 Homo sapiens
lipid phosphate phosphatase
-
 Homo sapiens
More c.f. EC 3.3.2.10 Homo sapiens
Nterm-phos
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0037
-
 IC50 is 0.0037 mM Homo sapiens sodium dodecyl sulfonate
0.0047
-
 IC50 is 0.0047 mM Homo sapiens octadecan-9-yl sulfate
0.005
-
 IC50 is 0.005 mM Homo sapiens taurolithocholic acid 3-sulfate
0.0052
-
 IC50 is 0.0052 mM Homo sapiens Sodium dodecyl sulfate
0.0059
-
 IC50 is 0.0059 mM Homo sapiens 10-(sulfooxy)octadecanoic acid
0.0096
-
 IC50 is 0.0096 mM Homo sapiens alpha-sulfostearic acid
0.0097
-
 IC50 is 0.0097 mM Homo sapiens (9E)-12-sulfonooxy-9-octadecenoic acid
0.013
-
 IC50 is 0.013 mM Homo sapiens dioctyl phenyl phosphonate
0.0175
-
 IC50 is 0.0175 mM Homo sapiens 9-hydroxy-10-(sulfooxy)octadecanoic acid
0.04
-
 IC50 is 0.040 mM Homo sapiens dodecyl phosphonic acid
0.073
-
 IC50 is 0.073 mM Homo sapiens alpha-hydroxyfarnesyl phosphonic acid
0.1
-
 IC50 is above 0.1 mM Homo sapiens 4-nitrophenyl sulfate
0.1
-
 IC50 is above 0.1 mM Homo sapiens taurocholic acid
0.1
-
 IC50 is above 0.1 mM Homo sapiens Estrone 3-sulfate
0.1
-
 IC50 is above 0.1 mM Homo sapiens N-acetyl-D-galactosamine 4-sulfate
0.1
-
 IC50 is above 0.1 mM Homo sapiens D-galactose 6-sulfate
0.1
-
 IC50 is above 0.1 mM Homo sapiens L-ascorbic acid 2-sulfate
0.1
-
 IC50 is above 0.1 mM Homo sapiens tetraisopropyl methylenediphosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens diethyl vinylphosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens diethyl benzoylphosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens diethyl cyclopropyl methylphosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens diethyl trans-cinnamyl phosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens diethyl 4-methylbenzyl phosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens diethyl allyl phosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens dibenzyl phosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens dimethyl 2-oxoheptyl phosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens diethyl ethylthiomethyl phosphonate
0.1
-
 IC50 is above 0.1 mM Homo sapiens (9Z)-12-sulfonooxy-9-octadecenoic acid