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Literature summary for 3.1.3.76 extracted from

  • Enayetallah, A.E.; Grant, D.F.
    Effects of human soluble epoxide hydrolase polymorphisms on isoprenoid phosphate hydrolysis (2006), Biochem. Biophys. Res. Commun., 341, 254-260.
    View publication on PubMed
Search for more literature for 3.1.3.76

Cloned(Commentary)

Cloned (Comment) Organism
expression of enzyme in insect cells using the baculovirus infection system Rattus norvegicus
expression of His-tagged wild-type and mutant enzymes in insect cells using the baculovirus infection system Homo sapiens

Protein Variants

Protein Variants Comment Organism
C154Y the mutant shows similar activity for isoprenoid phosphate compared to the wild-type enzyme Homo sapiens
R103C the mutant shows increased activity for isoprenoid phosphate compared to the wild-type enzyme Homo sapiens
R103C/R287Q the mutant shows similar activity for isoprenoid phosphate compared to the wild-type enzyme Homo sapiens
R287Q the mutant shows increased activity for isoprenoid phosphate compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition of the phosphatase activity by 1-cyclohexyl-3-dodecyl urea at 0.1 mM Homo sapiens
orthovanadate 65% inhibition of the phosphatase activity at 0.1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 farnesyl phosphate recombinant enzyme Homo sapiens
0.033
-
 geranylgeranyl diphosphate recombinant enzyme Homo sapiens
0.084
-
 farnesyl diphosphate recombinant enzyme Homo sapiens
0.124
-
 geranyl phosphate recombinant enzyme Homo sapiens
1.746
-
 geranylgeranyl phosphate recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Rattus norvegicus
Mg2+ dependent on, maximal activity at 0.1 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme by S-benzyl affinity chromatography with 4-fluorochalcone oxide as eluent Rattus norvegicus
recombinant His-tagged wild-type and mutant enzymes from insect cells by nickel affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently Homo sapiens
a
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 purified recombinant enzyme, substrate isopentenyl phosphate Homo sapiens
0.25
-
 purified recombinant enzyme, substrate geranyl phosphate Homo sapiens
0.49
-
 purified recombinant enzyme, substrate farnesyl phosphate Homo sapiens
0.53
-
 purified recombinant enzyme, substrate geranylgeranyl phosphate Homo sapiens
0.8
-
 purified recombinant enzyme, substrate geranyl phosphate Rattus norvegicus
0.85
-
 purified recombinant enzyme, substrate isopentenyl phosphate Rattus norvegicus
0.99
-
 purified recombinant enzyme, substrate farnesyl phosphate Rattus norvegicus
1.05
-
 purified recombinant enzyme, substrate geranylgeranyl phosphate Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + H2O
-
 Homo sapiens farnesol + phosphate
-
 ?
farnesyl phosphate + H2O
-
 Homo sapiens farnesol + phosphate
-
 ?
farnesyl phosphate + H2O
-
 Rattus norvegicus farnesol + phosphate
-
 ?
geranyl diphosphate + H2O
-
 Homo sapiens geraniol + phosphate
-
 ?
geranyl phosphate + H2O
-
 Homo sapiens geraniol + phosphate
-
 ?
geranyl phosphate + H2O
-
 Rattus norvegicus geraniol + phosphate
-
 ?
geranylgeranyl diphosphate + H2O
-
 Homo sapiens geranylgeraniol + phosphate
-
 ?
geranylgeranyl phosphate + H2O
-
 Homo sapiens geranylgeraniol + phosphate
-
 ?
geranylgeranyl phosphate + H2O
-
 Rattus norvegicus geranylgeraniol + phosphate
-
 ?
isopentenyl diphosphate + H2O
-
 Homo sapiens isopentenol + phosphate
-
 ?
isopentenyl phosphate + H2O
-
 Homo sapiens isopentenol + phosphate
-
 ?
isopentenyl phosphate + H2O
-
 Rattus norvegicus isopentenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
More c.f. EC 3.3.2.10 Homo sapiens
More c.f. EC 3.3.2.10 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 geranylgeranyl diphosphate recombinant enzyme Homo sapiens
0.25
-
 farnesyl diphosphate recombinant enzyme Homo sapiens
0.51
-
 geranyl phosphate recombinant enzyme Homo sapiens
0.71
-
 farnesyl phosphate recombinant enzyme Homo sapiens
1.85
-
 geranylgeranyl phosphate recombinant enzyme Homo sapiens