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Literature summary for 3.1.3.76 extracted from
- Polymorphisms in human soluble epoxide hydrolase: effects on enzyme activity, enzyme stability, and quarternary structure (2004), Arch. Biochem. Biophys., 427, 164-169.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene EPHX2, expression of His-tagged wild-type and mutant enzymes in Spodoptera frugiperda Sf21 cells using the baculovirus infection system | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C154Y | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 2.2, compared to the wild-type ratio of 1.0 | Homo sapiens |
| E470G | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 0.9, compared to the wild-type ratio of 1.0 | Homo sapiens |
| K55R | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.9, compared to the wild-type ratio of 1.0 | Homo sapiens |
| R103C | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.3, compared to the wild-type ratio of 1.0 | Homo sapiens |
| R103C/R287Q | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.2, compared to the wild-type ratio of 1.0 | Homo sapiens |
| R287Q | site-directed mutagenesis, the mutant shows the same hydrolase/phosphatase ratio of 1.0 as the wild-type, but reduced stability at 37°C | Homo sapiens |
| V422A | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 0.8, compared to the wild-type ratio of 1.0 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
4-nitrophenyl phosphate | pH 7.4, 37°C, recombinant wild-type allele MFA enzyme and mutant R103C | Homo sapiens |  |
| 1.2 | - |
4-nitrophenyl phosphate | pH 7.4, 37°C, recombinant mutants K55R and C154Y | Homo sapiens | |
| 1.3 | - |
4-nitrophenyl phosphate | pH 7.4, 37°C, recombinant mutant R287Q | Homo sapiens | |
| 2.5 | - |
4-nitrophenyl phosphate | pH 7.4, 37°C, recombinant mutant R103C/R287Q | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for phosphatase activity of the enzyme | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
gene EPHX2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Sf21 insect cells by nickel affinity chromatography | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate | the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.007 | - |
purified recombinant mutant R103C/R287Q, substrate 4-nitrophenyl phosphate | Homo sapiens |
| 0.015 | - |
purified recombinant mutant K55R, substrate 4-nitrophenyl phosphate | Homo sapiens |
| 0.016 | - |
purified recombinant mutant C154Y, substrate 4-nitrophenyl phosphate | Homo sapiens |
| 0.017 | - |
purified recombinant mutant R103C, substrate 4-nitrophenyl phosphate | Homo sapiens |
| 0.021 | - |
purified recombinant mutant R287Q, substrate 4-nitrophenyl phosphate | Homo sapiens |
| 0.029 | - |
purified recombinant wild-type allele MFA enzyme and mutant E470G, substrate 4-nitrophenyl phosphate | Homo sapiens |
| 0.03 | - |
purified recombinant mutant V422A, substrate 4-nitrophenyl phosphate | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the phosphatase activity of the enzyme is located at N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, Arg287 may be involved in formation of the quarternary enzyme structure | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EPXH2 | - |
Homo sapiens |
| More | c.f. EC 3.3.2.10 | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
inactivation rate constants for recombinant wild-type and mutant enzymes | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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