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Literature summary for 3.1.3.74 extracted from

  • Knobloch, G.; Jabari, N.; Stadlbauer, S.; Schindelin, H.; Koehn, M.; Gohla, A.
    Synthesis of hydrolysis-resistant pyridoxal 5-phosphate analogs and their biochemical and X-ray crystallographic characterization with the pyridoxal phosphatase chronophin (2015), Bioorg. Med. Chem., 23, 2819-2827 .
    View publication on PubMed
Search for more literature for 3.1.3.74

Crystallization (Commentary)

Crystallization (Comment) Organism
structure in presence of inhibitor [2-[5-hydroxy-4-(hydroxymethyl)-6-methylpyridin-3-yl]ethyl]phosphonic acid. The overall structure of murine PDXP remains unaltered by ligand binding Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
[(E)-2-(4-formyl-5-hydroxy-6-methylpyridin-3-yl)ethenyl]phosphonic acid compound increases the Km up to 6fold at 2 mM. The catalytic efficiency is reduced to 10% in the presence of 2 mM of the compound Mus musculus
[2-(4-formyl-5-hydroxy-6-methylpyridin-3-yl)ethyl]phosphonic acid compound increases the Km up to 6fold at 2 mM. The catalytic efficiency is reduced to 10% in the presence of 2 mM of the compound Mus musculus
[2-[5-hydroxy-4-(hydroxymethyl)-6-methylpyridin-3-yl]ethyl]phosphonic acid
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 pyridoxal 5'-phosphate pH 7.4, 22°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P60487
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxal 5'-phosphate + H2O
-
 Mus musculus pyridoxal + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 pyridoxal 5'-phosphate pH 7.4, 22°C Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.079
-
 pH 7.4, 22°C Mus musculus [2-(4-formyl-5-hydroxy-6-methylpyridin-3-yl)ethyl]phosphonic acid
0.246
-
 pH 7.4, 22°C Mus musculus [(E)-2-(4-formyl-5-hydroxy-6-methylpyridin-3-yl)ethenyl]phosphonic acid
1.3
-
 pH 7.4, 22°C Mus musculus [2-[5-hydroxy-4-(hydroxymethyl)-6-methylpyridin-3-yl]ethyl]phosphonic acid

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
50
-
 pyridoxal 5'-phosphate pH 7.4, 22°C Mus musculus