Literature summary for 3.1.3.74 extracted from

Kim, J.E.; Kim, D.W.; Kwak, S.E.; Kwon, O.S.; Choi, S.Y.; Kang, T.C.
Potential role of pyridoxal-5-phosphate phosphatase/chronopin in epilepsy (2008), Exp. Neurol., 211, 128-140.

Cloned(Commentary)

Cloned (Comment)	Organism
getting Tat-PLPP/CIN: amplification of PLPP/CIN via PCR and inserted into pGEM-T vector, subcloned into pET15b, transformation of BL21 Escherichia coli. getting PLPP: cDNA of PLPP amplified via PCR and cloned into pET15b, transformation of Escherichia coli JM109 (DE3). experiments with Sprague-Dawley (SD) rats (7 weeks old). The effect of PNP treatment on PLPP/CIN immunoreactivity in the rat hippocampus: vitamin B6 may not be directly involved in ADF/cofilin-mediated F-actin depolymerization in normal condition. Up-regulation of PLPP/CIN expression following status epilepticus may play an important role in the formation of epileptic hippocampus. Hippocampal excitability following transduction of Tat-PLPP/CIN: F-actin depolymerization induced by over-expression of PLPP/CIN may increase excitability ratio in the hippocampus via postsynaptic changes, not via impaired GABAergic inhibition (immunohistochemistry, optical density, enzyme activity, electrophysiological analysis)	Rattus norvegicus

Cloned (Comment)

Organism

getting Tat-PLPP/CIN: amplification of PLPP/CIN via PCR and inserted into pGEM-T vector, subcloned into pET15b, transformation of BL21 Escherichia coli. getting PLPP: cDNA of PLPP amplified via PCR and cloned into pET15b, transformation of Escherichia coli JM109 (DE3). experiments with Sprague-Dawley (SD) rats (7 weeks old). The effect of PNP treatment on PLPP/CIN immunoreactivity in the rat hippocampus: vitamin B6 may not be directly involved in ADF/cofilin-mediated F-actin depolymerization in normal condition. Up-regulation of PLPP/CIN expression following status epilepticus may play an important role in the formation of epileptic hippocampus. Hippocampal excitability following transduction of Tat-PLPP/CIN: F-actin depolymerization induced by over-expression of PLPP/CIN may increase excitability ratio in the hippocampus via postsynaptic changes, not via impaired GABAergic inhibition (immunohistochemistry, optical density, enzyme activity, electrophysiological analysis)

Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	localization of PLPP/CIN in the rat hippocampus: PLPP/CIN is mainly localized in postsynaptic compartments, but not in presynaptic compartments (immunohistochemistry)	Rattus norvegicus	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pyridoxal 5'-phosphate + H2O	Rattus norvegicus	directly dephosphorylates actin-depolymerizing factor (ADF)/cofilin, PLPP/CIN-mediated actin dynamics may play an important role in the changes of morphological properties and excitability of the epileptic hippocampus	pyridoxal + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	Sprague-Dawley rat	-

Purification (Commentary)

Purification (Comment)	Organism
lysed by French Press Cell and by sonication in binding buffer (5 mM imidazole, 500 mM NaCl, and 20 mMTris-HCl, pH 7.9)., Ni2+-IDA column, desalting with PD10 column	Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	approx. 42 units, control animals (saline-treated and without high-frequency stimulation), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance reduction at 390 nm (3 min)	Rattus norvegicus
additional information	-	approx. 48 units, PNPP/CIN, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance reduction at 390 nm (3 min)	Rattus norvegicus
additional information	-	approx. 50 units, control animals (saline-treated and without high-frequency stimulation), 1 week after status epilepticus, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance reduction at 390 nm (3 min)	Rattus norvegicus
additional information	-	approx. 62 units, Tat-PNPP/CIN, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance reduction at 390 nm (3 min)	Rattus norvegicus
additional information	-	approx. 65 units, control animals (saline-treated and without high-frequency stimulation), 6 weeks after statur epilepticus, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance reduction at 390 nm (3 min)	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyridoxal 5'-phosphate + H2O	-	Rattus norvegicus	pyridoxal + phosphate	-	?
pyridoxal 5'-phosphate + H2O	directly dephosphorylates actin-depolymerizing factor (ADF)/cofilin, PLPP/CIN-mediated actin dynamics may play an important role in the changes of morphological properties and excitability of the epileptic hippocampus	Rattus norvegicus	pyridoxal + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PLPP/CIN	-	Rattus norvegicus
pyridoxal-5´-phosphate (PLP)-phosphatase/chronophin	-	Rattus norvegicus