Cloned (Comment) | Organism |
---|---|
recombinant extracellular expression of Lilium longiflorum alkaline phytase in Pichia pastoris strain X33. For analysis of the influence of the native signal sequences on alpha-MF-directed secretion, three expression constructs are generated by inserting three forms of rLlAlp2 downstream of the alpha-MF secretion signal in pPICZaAvectors: 1. full length optimized rLlAlp2 sequence (pPICZaA-op-rLlAlp2), 2. truncated oprLlAlp2 without QKTEL at the C-terminus (pPICZaA-op-LlAlp2DC), and 3. truncated op-rLlAlp2 without the first 19 aa at the N-terminus and without QKTEL at the C-terminus (pPICZaA-oprLlAlp2DNDC). By switching from the strong, tightly regulated promoter PAOX1 to the weaker, constitutive promoter PGAP and optimizing culture conditions, an 8 to 10fold increase in extracellular expression of rLlALP2 is achieved, optimization of culture conditions for enhanced expression, method evaluation, overview | Lilium longiflorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Lilium longiflorum | phosphate cleavage position is not determined | myo-inositol pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lilium longiflorum | Q0GYS2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pollen | - |
Lilium longiflorum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined | Lilium longiflorum | myo-inositol pentakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline phytase | - |
Lilium longiflorum |
LlALP2 | - |
Lilium longiflorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lilium longiflorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Lilium longiflorum |