Literature summary for 3.1.3.72 extracted from

Yang, M.; Johnson, S.C.; Murthy, P.P.
Enhancement of alkaline phytase production in Pichia pastoris: influence of gene dosage, sequence optimization and expression temperature (2012), Protein Expr. Purif., 84, 247-254.

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Application

Application	Comment	Organism
synthesis	transgenic expression in Pichia pastoris. Increasing isoform Alp2 copy number is detrimental to heterologous expression, clones with one copy of wild-type Alp2 produce the highest activity, clones with two, four and seven or more copies produce 70%, 25% and 10% respectively, of enzyme activity. Use of a sequence-optimized Alp2 gene increases the yield of the active enzyme by 25-50% in one/two copy clones. Reducing the temperature during heterologous expression leads to increases of 1.2-20fold suggesting that protein folding and post-translational processes may be the dominant factors limiting ALP2 expression	Lilium longiflorum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Lilium longiflorum

Organism

Organism	UniProt	Comment	Textmining
Lilium longiflorum	Q0GYS2	isoform Alp2	-

Synonyms

Synonyms	Comment	Organism
Alp2	-	Lilium longiflorum

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Release 2023.1 (January 2023)