Literature summary for 3.1.3.72 extracted from

Garchow, B.G.; Jog, S.P.; Mehta, B.D.; Monosso, J.M.; Murthy, P.P.
Alkaline phytase from Lilium longiflorum: Purification and structural characterization (2006), Protein Expr. Purif., 46, 221-232.

Search for more literature for 3.1.3.72

Application

Application	Comment	Organism
agriculture	useful as food and animal feed supplement	Lilium longiflorum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Na+	-	Lilium longiflorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
118000	-	by Ferguson plot analysis	Lilium longiflorum

Organism

Organism	UniProt	Comment	Textmining
Lilium longiflorum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
selective precipitation by heat and ammonium sulfate followed by anion exchange and chromatofocusing chromatography and gel filtration, purified 3300fold, to homogeneity	Lilium longiflorum

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	pollen grain	Lilium longiflorum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.017	-	crude extract	Lilium longiflorum
56	-	3300fold purified enzyme	Lilium longiflorum

Storage Stability

Storage Stability	Organism
-80°C, stable for 4 weeks	Lilium longiflorum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O	-	Lilium longiflorum	myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	in the range of 52000-55000, SDS-PAGE	Lilium longiflorum

Synonyms

Synonyms	Comment	Organism
alkaline phytase	-	Lilium longiflorum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Lilium longiflorum

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Release 2023.1 (January 2023)