Literature summary for 3.1.3.71 extracted from

DiDonato, M.; Krishna, S.S.; Schwarzenbacher, R.; McMullan, D.; Agarwalla, S.; Brittain, S.M.; Miller, M.D.; Abdubek, P.; Ambing, E.; Axelrod, H.L.; Canaves, J.M.; Chiu, H.; Deacon, A.M.; Duan, L.; Elsliger, M.; Godzik, A.; Grzechnik, S.K.; Hale, J.; et.al.
Crystal structure of 2-phosphosulfolactate phosphatase (ComB) from Clostridium acetobutylicum at 2.6.A. resolution reveals a new fold with a novel active site (2006), Proteins Struct. Funct. Bioinform., 65, 771-776.

Cloned(Commentary)

Cloned (Comment)	Organism
into plasmid pMH4, expressed in Escherichia coli strain DL41	Clostridium acetobutylicum

Crystallization (Comment)	Organism
by the nanodroplet vapor diffusion method, at 2.6 A resolution. Belongs to space group C2221 with unit cell parameters a = 46.70, b = 69.19, c = 453.52 A. Model of three ComB monomers (residues 1-235 for chains A, B, and C), three (2R)-3-sulfolactate molecules, and 93 water molecules in the asymmetric unit. ComB is composed of 10 beta-strands, nine alpha-helices and five 310-helices	Clostridium acetobutylicum

Metals/Ions	Comment	Organism	Structure
Mg2+	contains two Mg2+ ions in the asymmetric unit	Clostridium acetobutylicum

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26010	-	gel filtration	Clostridium acetobutylicum

Organism	UniProt	Comment	Textmining
Clostridium acetobutylicum	Q97E82	-	-

Purification (Comment)	Organism
on Ni2+ resin and by gel filtration	Clostridium acetobutylicum

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	residues involved in catalysis and/or binding include Arg29, Asp26, Asp154, Asp155, Thr103, Thr147, and Asn104	Clostridium acetobutylicum	?	-	?

Subunits	Comment	Organism
dimer	crystallography	Clostridium acetobutylicum

Synonyms	Comment	Organism
ComB	-	Clostridium acetobutylicum