Any feedback?
Literature summary for 3.1.3.71 extracted from
- Identification of coenzyme M biosynthetic 2-phosphosulfolactate phosphatase. A member of a new class of Mg2+-dependent acid phosphatases (2001), Eur. J. Biochem., 268, 5176-5188.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-carboxyethylphosphonate | weak, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii |  |
| Ca2+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
| Cd2+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
| Cu+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
| Fe2+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
| Fe3+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
| Mg2+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
| vanadate | - |
Methanocaldococcus jannaschii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
(S)-phosphomalate | - |
Methanocaldococcus jannaschii | |
| 0.011 | - |
(R)-phosphomalate | - |
Methanocaldococcus jannaschii | |
| 0.034 | - |
Phosphoglycolate | - |
Methanocaldococcus jannaschii | |
| 0.063 | - |
rac-phosphosulfolactate | - |
Methanocaldococcus jannaschii | |
| 0.148 | - |
(S)-phospholactate | - |
Methanocaldococcus jannaschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can fully substitute for Mg2+ | Methanocaldococcus jannaschii | |
| Cu2+ | weak activation | Methanocaldococcus jannaschii | |
| Mg2+ | dependent on, at a concentration approximately equimolar with substrate | Methanocaldococcus jannaschii | |
| Mn2+ | can fully substitute for Mg2+ | Methanocaldococcus jannaschii | |
| Ni2+ | can fully substitute for Mg2+ | Methanocaldococcus jannaschii | |
| Zn2+ | can fully substitute for Mg2+ | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-3-sulfolactate + H2O | Methanocaldococcus jannaschii | the enzyme catalyzes the second step in biosynthesis of coenzyme M | 3-sulfolactate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
| Methanosarcina thermophila | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 48 | - |
rac-phosphosulfolactate | Methanocaldococcus jannaschii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, in presence of 50% v/v glycerol, 25% loss of activity after 3 weeks | Methanocaldococcus jannaschii |
| 4°C, 7% loss of activity after 3 weeks | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-phospholactate + H2O | - |
Methanocaldococcus jannaschii | lactate + phosphate | - |
? | |
| (R)-phosphomalate + H2O | - |
Methanocaldococcus jannaschii | malate + phosphate | - |
? | |
| (S)-2-phospholactate + H2O | no activity with (R)-phospholactate | Methanocaldococcus jannaschii | lactate + phosphate | - |
? | |
| (S)-2-phosphomalate + H2O | - |
Methanocaldococcus jannaschii | malate + phosphate | - |
? | |
| 2-phospho-3-sulfolactate + H2O | the enzyme catalyzes the second step in biosynthesis of coenzyme M | Methanocaldococcus jannaschii | 3-sulfolactate + phosphate | - |
? | |
| additional information | in presence of (S)-lactate the histidine-tagged enzyme can dephosphorylate phosphoglycolate and produce 2-phospholactate | Methanocaldococcus jannaschii | ? | - |
? | |
| phosphoglycolate + H2O | - |
Methanocaldococcus jannaschii | glycolate + phosphate | - |
? | |
| rac-2-phosphosulfolactate + H2O | - |
Methanocaldococcus jannaschii | sulfolactate + phosphate | - |
? | |
| rac-2-phosphosulfolactate + H2O | - |
Methanosarcina thermophila | sulfolactate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate | Methanocaldococcus jannaschii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 85 | 65°C: about 50% of maximal activity, 85°C: about 70% of maximal activity, polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate | Methanocaldococcus jannaschii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | pH 4.5: about 80% of maximal activity, pH 7.0: about 55%of maximal activity, polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate | Methanocaldococcus jannaschii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
