Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.70 extracted from

  • Empadinhas, N.; Albuquerque, L.; Henne, A.; Santos, H.; Da Costa, M.
    The bacterium Thermus thermophilus, like hyperthermophilic archaea, uses a two-step pathway for the synthesis of mannosylglycerate (2003), Appl. Environ. Microbiol., 69, 3272-3279 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
2-O-(alpha-D-mannosyl)-3-phosphoglycerate pH 7.6, 70°C Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme Thermus thermophilus
Mg2+ activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme Thermus thermophilus
Mn2+ activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme Thermus thermophilus
additional information Ca2+, Sr2+, Ba2+, Cu2+, and Zn2+, have no effect on enzyme activity at any concentration Thermus thermophilus
Ni2+ activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28100
-
calculated from sequence Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q84B23
-
-
Thermus thermophilus DSM 7039 Q84B23
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-(alpha-D-mannosyl)-3-phosphoglycerate + H2O high substrate specificity, 2-O-(alpha-D-mannosyl)-3-phosphoglycerate is the only substrate dephosphorylated by the enzyme Thermus thermophilus 2-O-(alpha-D-mannosyl)-D-glycerate + phosphate
-
?
2-O-(alpha-D-mannosyl)-3-phosphoglycerate + H2O high substrate specificity, 2-O-(alpha-D-mannosyl)-3-phosphoglycerate is the only substrate dephosphorylated by the enzyme Thermus thermophilus DSM 7039 2-O-(alpha-D-mannosyl)-D-glycerate + phosphate
-
?

Synonyms

Synonyms Comment Organism
MPGP
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90 95
-
Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 103 30°C: about 15% of maximal activity, 103°C: about 70% of maximal activity Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life: 25 min Thermus thermophilus
100
-
half-life: 2 min Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Thermus thermophilus