Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
2-O-(alpha-D-mannosyl)-3-phosphoglycerate | pH 7.6, 70°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme | Thermus thermophilus | |
Mg2+ | activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme | Thermus thermophilus | |
Mn2+ | activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme | Thermus thermophilus | |
additional information | Ca2+, Sr2+, Ba2+, Cu2+, and Zn2+, have no effect on enzyme activity at any concentration | Thermus thermophilus | |
Ni2+ | activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28100 | - |
calculated from sequence | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q84B23 | - |
- |
Thermus thermophilus DSM 7039 | Q84B23 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-(alpha-D-mannosyl)-3-phosphoglycerate + H2O | high substrate specificity, 2-O-(alpha-D-mannosyl)-3-phosphoglycerate is the only substrate dephosphorylated by the enzyme | Thermus thermophilus | 2-O-(alpha-D-mannosyl)-D-glycerate + phosphate | - |
? | |
2-O-(alpha-D-mannosyl)-3-phosphoglycerate + H2O | high substrate specificity, 2-O-(alpha-D-mannosyl)-3-phosphoglycerate is the only substrate dephosphorylated by the enzyme | Thermus thermophilus DSM 7039 | 2-O-(alpha-D-mannosyl)-D-glycerate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MPGP | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 95 | - |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 103 | 30°C: about 15% of maximal activity, 103°C: about 70% of maximal activity | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 25 min | Thermus thermophilus |
100 | - |
half-life: 2 min | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermus thermophilus |