Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Debaryomyces hansenii |
Protein Variants | Comment | Organism |
---|---|---|
D201N | 8.2% relative activity compared to the wild type enzyme | Debaryomyces hansenii |
D363N | 6.2% relative activity compared to the wild type enzyme | Debaryomyces hansenii |
E133Q | 4.8% relative activity compared to the wild type enzyme | Debaryomyces hansenii |
E301K | strongly reduced activity compared to the wild type enzyme | Debaryomyces hansenii |
T206A | 5.6% relative activity compared to the wild type enzyme | Debaryomyces hansenii |
V131A | strongly reduced activity compared to the wild type enzyme | Debaryomyces hansenii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | - |
Debaryomyces hansenii | |
Na+ | - |
Debaryomyces hansenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01704 | - |
adenosine 3',5'-bisphosphate | wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
0.02646 | - |
adenosine 3',5'-bisphosphate | mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
0.07299 | - |
adenosine 3',5'-bisphosphate | mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Debaryomyces hansenii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Debaryomyces hansenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine 3',5'-bisphosphate + H2O | - |
Debaryomyces hansenii | adenosine 5'-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3(2),5-bisphosphate nucleotidase | - |
Debaryomyces hansenii |
BPntase | - |
Debaryomyces hansenii |
Dhal2p | - |
Debaryomyces hansenii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
adenosine 3',5'-bisphosphate | mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
1.465 | - |
adenosine 3',5'-bisphosphate | mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
1.921 | - |
adenosine 3',5'-bisphosphate | wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
2.94 | - |
adenosine 3',5'-bisphosphate | wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
6.08 | - |
adenosine 3',5'-bisphosphate | mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2.4 | - |
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | Li+ | |
180 | - |
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | Na+ |