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Literature summary for 3.1.3.7 extracted from

  • Aggarwal, M.; Kishan, K.V.; Mondal, A.K.
    Creation of salt-insensitive 3(2),5-bisphosphate nucleotidase by modeling and mutagenesis approach (2008), Arch. Biochem. Biophys., 469, 174-183.
    View publication on PubMed
Search for more literature for 3.1.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Debaryomyces hansenii

Protein Variants

Protein Variants Comment Organism
D201N 8.2% relative activity compared to the wild type enzyme Debaryomyces hansenii
D363N 6.2% relative activity compared to the wild type enzyme Debaryomyces hansenii
E133Q 4.8% relative activity compared to the wild type enzyme Debaryomyces hansenii
E301K strongly reduced activity compared to the wild type enzyme Debaryomyces hansenii
T206A 5.6% relative activity compared to the wild type enzyme Debaryomyces hansenii
V131A strongly reduced activity compared to the wild type enzyme Debaryomyces hansenii

Inhibitors

Inhibitors Comment Organism Structure
Li+
-
 Debaryomyces hansenii
Na+
-
 Debaryomyces hansenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01704
-
 adenosine 3',5'-bisphosphate wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
0.02646
-
 adenosine 3',5'-bisphosphate mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
0.07299
-
 adenosine 3',5'-bisphosphate mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii

Organism

Organism UniProt Comment Textmining
Debaryomyces hansenii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Debaryomyces hansenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 3',5'-bisphosphate + H2O
-
 Debaryomyces hansenii adenosine 5'-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
3(2),5-bisphosphate nucleotidase
-
 Debaryomyces hansenii
BPntase
-
 Debaryomyces hansenii
Dhal2p
-
 Debaryomyces hansenii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.069
-
 adenosine 3',5'-bisphosphate mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
1.465
-
 adenosine 3',5'-bisphosphate mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
1.921
-
 adenosine 3',5'-bisphosphate wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
2.94
-
 adenosine 3',5'-bisphosphate wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
6.08
-
 adenosine 3',5'-bisphosphate mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4
-
 wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii Li+
180
-
 wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii Na+