BRENDA - Enzyme Database show
show all sequences of 3.1.3.69

Production of the compatible solute alpha-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum

Roenneke, B.; Rosenfeldt, N.; Derya, S.M.; Novak, J.F.; Marin, K.; Kraemer, R.; Seibold, G.M.; Microb. Cell Fact. 17, 94 (2018)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
plasmid-bound expression of glucosylglycerol synthase and glucosylglycerol phosphatase from Synechocystis sp. PCC 6803 enables alpha-D-glucosylglycerol synthesis exclusively in osmotically stressed cells of Corynebacterium glutamicum. Upon deletion of both the glgA encoded glycogen synthase and the otsA encoded trehalose-6-phosphate synthase, the alpha-D-glucosylglycerol concentration in culture supernatants is increased from 0.5 mM in to 2.9 mM. Upon nitrogen limitation, transgenic Corynebacterium produces more than 10 mM alpha-D-glucosylglycerol (about 2 g/l)
Synechocystis sp. PCC 6803
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Synechocystis sp. PCC 6803
Q55034
-
-
Application (protein specific)
Application
Commentary
Organism
synthesis
plasmid-bound expression of glucosylglycerol synthase and glucosylglycerol phosphatase from Synechocystis sp. PCC 6803 enables alpha-D-glucosylglycerol synthesis exclusively in osmotically stressed cells of Corynebacterium glutamicum. Upon deletion of both the glgA encoded glycogen synthase and the otsA encoded trehalose-6-phosphate synthase, the alpha-D-glucosylglycerol concentration in culture supernatants is increased from 0.5 mM in to 2.9 mM. Upon nitrogen limitation, transgenic Corynebacterium produces more than 10 mM alpha-D-glucosylglycerol (about 2 g/l)
Synechocystis sp. PCC 6803
Other publictions for EC 3.1.3.69
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751484
Roenneke
Production of the compatible ...
Synechocystis sp. PCC 6803
Microb. Cell Fact.
17
94
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
750630
Song
The response regulator Slr158 ...
Synechocystis sp. PCC 6803
Front. Microbiol.
8
1176
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
692870
Hagemann
The plant-associated bacterium ...
Stenotrophomonas rhizophila
J. Bacteriol.
190
5898-5906
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170829
Hagemann
The gene stpA from Synechocyst ...
Synechocystis sp.
J. Bacteriol.
179
1727-1733
1997
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170828
Hagemann
Characterization of a glucosyl ...
Synechocystis sp.
Arch. Microbiol.
166
83-91
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656477
Schoor
-
Non-radiometric assay for gluc ...
Synechocystis sp.
J. Microbiol. Methods
27
139-145
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170827
Hagemann
-
Activation and pathway of gluc ...
Synechocystis sp.
Microbiology
140
1427-1431
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-