BRENDA - Enzyme Database
show all sequences of 3.1.3.68

Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols

Toivari, M.H.; Ruohonen, L.; Miasnikov, A.N.; Richard, P.; Penttila, M.; Appl. Environ. Microbiol. 73, 5471-5476 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression of DOG1 in Saccharomyces cerevisiae transketolase-deficient strain H1520 containing the integrated xylitol dehydrogenase-encoding gene (XYL2) gene increases the excretion of ribitol (by 1.6fold) and D-ribose (by 3.8fold) compared to the strain lacking DOG1
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Saccharomyces cerevisiae
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
2-deoxy-glucose 6-phosphate (100%), D-ribulose 5-phosphate (7%), D-ribose 5-phosphate (42%), D-xylulose 5-phosphate (15%)
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-Deoxy-D-glucose 6-phosphate + H2O
-
677697
Saccharomyces cerevisiae
2-Deoxy-D-glucose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
-
677697
Saccharomyces cerevisiae
D-ribose + phosphate
-
-
-
?
D-ribulose 5-phosphate + H2O
-
677697
Saccharomyces cerevisiae
D-ribulose + phosphate
-
-
-
?
D-xylulose 5-phosphate + H2O
-
677697
Saccharomyces cerevisiae
D-xylulose + phosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
2-deoxy-glucose 6-phosphate phosphatase
-
Saccharomyces cerevisiae
DOG1
-
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of DOG1 in Saccharomyces cerevisiae transketolase-deficient strain H1520 containing the integrated xylitol dehydrogenase-encoding gene (XYL2) gene increases the excretion of ribitol (by 1.6fold) and D-ribose (by 3.8fold) compared to the strain lacking DOG1
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
2-deoxy-glucose 6-phosphate (100%), D-ribulose 5-phosphate (7%), D-ribose 5-phosphate (42%), D-xylulose 5-phosphate (15%)
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-Deoxy-D-glucose 6-phosphate + H2O
-
677697
Saccharomyces cerevisiae
2-Deoxy-D-glucose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
-
677697
Saccharomyces cerevisiae
D-ribose + phosphate
-
-
-
?
D-ribulose 5-phosphate + H2O
-
677697
Saccharomyces cerevisiae
D-ribulose + phosphate
-
-
-
?
D-xylulose 5-phosphate + H2O
-
677697
Saccharomyces cerevisiae
D-xylulose + phosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 3.1.3.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750667
Izawati
Evaluation on the effectivene ...
Saccharomyces cerevisiae
Front. Plant Sci.
6
727
2015
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1
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677697
Toivari
Metabolic engineering of Sacch ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
73
5471-5476
2007
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4
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1
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25141
Randez-Gil
DOGR1 and DOGR2: two genes fro ...
Saccharomyces cerevisiae
Yeast
11
1233-1240
1995
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2
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8
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2
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1
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25142
Randez-Gil
The expression of a specific 2 ...
Saccharomyces cerevisiae
Curr. Genet.
28
101-107
1995
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3
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25143
Sanz
Molecular characterization of ...
Saccharomyces cerevisiae
Yeast
10
1195-1202
1994
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1
1
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2
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25144
Martin
Characterization of a phosphat ...
Saccharomyces cerevisiae 10a12-13, Saccharomyces cerevisiae, Saccharomyces cerevisiae PM-II
FEBS Lett.
83
245-248
1977
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