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Literature summary for 3.1.3.67 extracted from

  • Dey, N.; Crosswell, H.E.; De, P.; Parsons, R.; Peng, Q.; Su, J.D.; Durden, D.L.
    The protein phosphatase activity of PTEN regulates SRC family kinases and controls glioma migration (2008), Cancer Res., 68, 1862-1871.
    View publication on PubMed
Search for more literature for 3.1.3.67

Cloned(Commentary)

Cloned (Comment) Organism
glioma cell lines U-87MG and U-373MG are stably transfected with wildtype PTEN or catalytically altered mutants of PTEN Mus musculus

Protein Variants

Protein Variants Comment Organism
G129E protein phosphatase-active, lipid phosphatase inactive mutant, expression of G129E mutant of PTEN in U-87MG cells has no effect on the phosphorylation status of protein kinase B, the mutant displays 7% of wildtype lipid phosphatidylinositol 3,4,5-trisphosphate-phosphatase activitiy and 65% wild type protein phosphatase activitiy in glioma cells Mus musculus
R130M kinase dead mutant, expression of R130M mutant of PTEN in U-87MG cells has no effect on the phosphorylation status of protein kinase B, the mutant displays 5% of wildtype lipid phosphatidylinositol 3,4,5-trisphosphate-phosphatase activitiy and 12% of wild-type protein phosphatase activitiy in glioma cells Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-myo-phosphatidylinositol 3,4,5-trisphosphate + H2O Mus musculus
-
 D-myo-phosphatidylinositol 4,5-bisphosphate + phosphate
-
 ?
additional information Mus musculus most of the tumor suppressor function of PTEN has been attributed to its ability to dephosphorylate the second messenger, phosphatidylinositol 3,4,5-triphosphate, resulting in the biological control of the phosphatidylinositol 3-kinase/protein kinase B pathway ?
-
 ?
additional information Mus musculus PTEN controls FYN kinase activity through its protein phosphatase function in glioma cells ?
-
 ?
additional information Mus musculus PTEN controls vitronectin-mediated migration through its protein phosphatase activity in glioma cells ?
-
 ?
additional information Mus musculus the protein phosphatase activity of PTEN regulates src family kinases and controls glioma migration ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 mice heterozygous for PTEN (+/-) on the C57BL6 genetic background
-

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte cell line cultures are established from wildtype, PTEN heterozygous (+/-) mice and PTEN and Fyn kinase double heterozygous (+/-) mice Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-phosphatidylinositol 3,4,5-trisphosphate + H2O
-
 Mus musculus D-myo-phosphatidylinositol 4,5-bisphosphate + phosphate
-
 ?
additional information most of the tumor suppressor function of PTEN has been attributed to its ability to dephosphorylate the second messenger, phosphatidylinositol 3,4,5-triphosphate, resulting in the biological control of the phosphatidylinositol 3-kinase/protein kinase B pathway Mus musculus ?
-
 ?
additional information PTEN controls FYN kinase activity through its protein phosphatase function in glioma cells Mus musculus ?
-
 ?
additional information PTEN controls vitronectin-mediated migration through its protein phosphatase activity in glioma cells Mus musculus ?
-
 ?
additional information the protein phosphatase activity of PTEN regulates src family kinases and controls glioma migration Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
PTEN
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus