Literature summary for 3.1.3.67 extracted from

Hoshino, Y.; Nishimura, K.; Sumpio, B.E.
Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain (2007), J. Cell. Biochem., 100, 515-526.

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Protein Variants

Protein Variants	Comment	Organism
380-385A	in endothelial cells transfected with 380-385A (mutation on the PTEN phosphorylation site) phosphatidylinositol 3,4,5-trisphosphate immunofluorescence intensity is than in wildtype or G129R transfected cells. Phosphatidylinositol 3,4,5-trisphosphate intensity in endothelial cells transfected with 380385A is weak but gradually increases to a maximum at 240 min after cyclic strain stimulation by 1.48fold relative to static condition	Bos taurus
G129R	in endothelial cells transfected with G129R, that lacks both protein and lipid phosphatase activities, phosphatidylinositol 3,4,5-trisphosphate immunofluorescence intensity is higher than transfection with wildtype PTEN. phosphatidylinositol 3,4,5-trisphosphate immunofluorescence in endothelial cells transfected with G129R rapidly increases and is maximum at 60 min after cyclic strain stimulation by 2.14fold relative to static condition	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	PTEN is inhibited by cyclic strain: cyclic strain increases phosphatidylinositol 3,4,5-trisphosphate immunofluorescence and peaks at 60 min after cyclic strain stimulation by 1.93fold in endothelial cells	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	exposure of bovine aortic endothelial cells to cyclic strain results in a time-dependent PTEN phosphorylation. Maximal phosphorylation occurres at 10 min after the cyclic strain stimulation by 2.21fold relative to static condition	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
endothelial cell	aortic endothelial cells, no change in PTEN protein expression in endothelial cells exposed to up to 24 h of cyclic strain	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain. Inhibition of PTEN activity has no effect on cyclic strain-mediated cell proliferation but inhibits cyclic strain mediated suppression of apoptosis	Bos taurus

Synonyms

Synonyms	Comment	Organism
MMAC1/TEP1	-	Bos taurus
PTEN	-	Bos taurus

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Release 2023.1 (January 2023)