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Literature summary for 3.1.3.66 extracted from

  • Thole, J.M.; Vermeer, J.E.; Zhang, Y.; Gadella, T.W.; Nielsen, E.
    Root hair defective4 encodes a phosphatidylinositol-4-phosphate phosphatase required for proper root hair development in Arabidopsis thaliana (2008), Plant Cell, 20, 381-395.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.66

Cloned(Commentary)

Cloned (Comment) Organism
wild-type RHD4 and the mutant rhd4-2 N-terminal catalytic domains expressed from Escherichia coli Rosetta cells or BL21 cells as GST fusion proteins. EYFP-wild-type fusion protein transformed into Arabidopsis under control of the native RHD4 promoter Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
G449S rhd4-2 mutant containing a mutation in motif VII of the SAC phosphatase domain, reduces the phosphatidylinositol-4-phosphate phosphatase activity to residual levels Arabidopsis thaliana
additional information rhd4-1 mutant, mutation results in root hairs that are shorter and randomly form bulges along their length. Stochastic loss and recovery of the RabA4b compartment in the tips of growing root hairs, altered subcellular distribution of phosphatidylinositol-4-phosphate within the root hair cell. EYFPRHD4 is capable of rescuing the rhd4-1 mutant phenotype Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane RHD4 is selectively recruited to RabA4-blabeled membranes that are involved in polarized expansion of root hair cells. In conjunction with the phosphoinositide kinase PI-4Kb1, RHD4 regulates the accumulation of phosphatidylinositol-4-phosphate on membrane compartments at the tips of growing root hairs Arabidopsis thaliana 16020
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Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9C5G5
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Purification (Commentary)

Purification (Comment) Organism
by gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root hair
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 Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylinositol-4-phosphate + H2O prefered substrate Arabidopsis thaliana ?
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 ?

Synonyms

Synonyms Comment Organism
phosphatidylinositol-4-phosphate phosphatase
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 Arabidopsis thaliana
RHD4
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 Arabidopsis thaliana
Sac1p-like phosphoinositide phosphatase
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 Arabidopsis thaliana